湖南省怀化地区白纹伊蚊核糖体18S rRNA和线粒体cox1基因的序列测定及分析

doi:10.16431/j.cnki.1671-7236.2021.08.006

Abstract

Abstract: The aim of this study was to explore the gene variation, genetic diversity and phylogenetic relationships of Aedes albopictus from Huaihua district, Hunan province.A total of 30 Aedes albopictus from Huaihua district were captured to use as research target.The ribosomal DNA 18S rRNA and cytochrome c oxidase subunit 1(cox1) gene sequences were amplified by PCR method, and then their gene variation, genetic diversity and phylogenetic relationships were analyzed, respectively.The results showed that the 18S rRNA and cox1 gene sequences of all samples was 489 and 1 004 bp, respectively.Based on 18S rRNA gene sequences, the intra-species differences of Aedes albopictus from Huaihua district were 0-2.9%, and the inter-species differences were 28.74%-61.46%.Based on cox1 gene sequences, the intra-species differences of Aedes albopictus from Huaihua district were 0-0.2%, and the inter-species differences were 6.08%-12.65%.The results of phylogenetic tree based on 18S rRNA and cox1 gene sequences showed that all samples of Aedes albopictus from Huaihua district together located in the same clade.The results of this study indicated there were a certain degree of genetic variation and genetic diversity within all samples of Aedes albopictus from Huaihua district, but the phylogenetic relationships were more closer among them, there was lower intra-species differences and higher inter-species differences between 18S rRNA and cox1 gene sequences, and 18S rRNA and cox1 gene could be used as an ideal genetic marker for the identification of Aedes albopictus.

Key words: Aedes albopictus; 18S rRNA; cox1 gene; genetic diversity; biological evolution

CLC Number:

S813.3

LI Zhongbo, HOU Qianghong. Sequencing and Sequence Analysis of Ribosomal 18S rRNA and Mitochondrial cox1 Gene of Aedes albopictus in Huaihua District,Hunan Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2736-2745.

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Sequencing and Sequence Analysis of Ribosomal 18S rRNA and Mitochondrial cox1 Gene of Aedes albopictus in Huaihua District,Hunan Province

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