基于mtDNA Cytb基因的马可·波罗盘羊遗传多样性与起源进化分析

doi:10.16431/j.cnki.1671-7236.2021.05.019

Abstract

Abstract: In order to further understand the genetic evolutionary relationship of Ovis ammon polii and domestic sheep,in this study,DNA of 20 Ovis ammon polii was extracted and mitochondrial DNA (mtDNA) cytochrome b (Cytb) gene was amplified by PCR.The mtDNA Cytb gene sequence in GenBank was downloaded,and the phylogenetic tree was constructed by Neighbor-Joining,so as to clarify the genetic diversity and evolution of wild sheep and domestic sheep.The results showed that the mtDNA Cytb gene of Ovis ammon polii was 1 140 bp,containing 58.3% A and T bases.There were 10 haplotypes,and the average haplotype diversity (Hd) was 0.884.There were 122 SNPs,accounting for 2.35% of the total nucleotide.Among them,there were 2 single polymorphic sites and 120 simple information sites,all of which were two-base mutations.The frequency of conversion was higher than that of inversion.The mean nucleotide diversity (pi) was 0.02090.The mean nucleotide difference (K) was 23.826.The results indicated that the mtDNA Cytb region of Ovis ammon polii was rich in haplotype and nucleotide genetic diversity.Phylogenetic analysis showed that wild sheep were divided into three distinct branches,among which Ovis ammon polii formed a single branch,and the genetic distance with Ovis ammon hodgsoni was the closest,and the genetic distance with Ovis ammon karelini, Ovis ammon darwini was the furthest,the domestic sheep breeds was closely related to Ovis musimon,but far from Ovis ammon polii.The results showed that Ovis ammon polii had a low contribution to the origin and evolution of domestic sheep,which supported the judgement that Ovis musimon might be the ancestor of domestic sheep.

Key words: Ovis ammon polii; mtDNA Cytb gene; genetic diversity; phylogenetic evolution

CLC Number:

S826

XIAO Yi, WANG Mengwen, ZHANG Xiuying, GULZAR Abdukerim, HAN Jianlin, WANG Yutao. Genetic Diversity,Origin and Evolution Analysis of Ovis ammon polii Based on mtDNA Cytb Gene[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1681-1690.

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Genetic Diversity,Origin and Evolution Analysis of Ovis ammon polii Based on mtDNA Cytb Gene

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