鸡Wnt3a基因组织表达差异与SNPs对鸡毛囊密度性状的遗传效应

doi:10.16431/j.cnki.1671-7236.2021.05.018

Abstract

Abstract: Wnt family member 3a (Wnt3a) gene is one of the important members in the Wnt signaling pathway,and the Wnt signaling pathway plays important roles in the development of animal skin and feather follicles.In this study,Wnt3a was selected as a candidate gene to detect the mRNA expression pattern for different tissues and the genetic effect of single nucleotide polymorphisms sites (SNPs) on feather follicle density in chicken.Real-time quantitative PCR was used to detect the mRNA expression profiles among different tissues.PCR amplification and direct sequencing technologies were used to identify the SNPs of the Wnt3a gene and analyze the difference of feather follicle density among different SNP genotypes.Furthermore,the Wnt3a mRNA expression difference of skin tissue between different chicken breeds was detected by Real-time-quantitative PCR.The results showed that the mRNA expression of skin was significantly higher than that of other tissues (P<0.05),the following tissues were liver,testis,ovary and hypothalamus,and heart,chest muscle,pituitary gland and spleen were the lowest.This study identified 3 SNPs,they were g.2587569 G>A,g.2555812 T>C and g.2555377 T>C,located in exons 2 and 3,and intron 3,respectively.Chi-square test showed that the genotype distribution of these SNPs had deviated significantly from Hardy-Weinberg equilibrium.Population genetic parameters analysis showed that g.2555812 T>C was moderately polymorphic,the number of effective alleles was 1.7,which indicated that the degree of variation in the population was higher than other SNPs.The correlation analysis between SNPs and feather follicle density showed that CC genotype at g.2555377 T>C were significantly higher than that of TT and CT genotypes (P<0.05).The mRNA expression level of the Wnt3a gene in skin tissues was significantly different between varieties,which showed a significant difference in feather density (P<0.05).The results of the study would provide a reference for further analyzing the role of the Wnt3a gene in chicken skin and feather follicles development and screening of molecular markers that contribute to feather follicle density traits.

Key words: chicken; Wnt3a gene; skin feather follicle; SNP; mRNA expression

CLC Number:

S834⁺.8

SHU Jingting, JI Gaige, TU Yunjie, ZHANG Ming, JU Xiaojun, SHAN Yanju, LIU Yifan, ZOU Jianmin. Tissue Expression Analysis of Wnt3a Gene and Genetic Effects of SNPs on Feather Follicle Density in Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1672-1680.

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Tissue Expression Analysis of Wnt3a Gene and Genetic Effects of SNPs on Feather Follicle Density in Chicken

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