不同地区褐黄血蜱的基因变异及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2021.06.028

Abstract

Abstract: To explore the genetic variation and phylogenetic evolution of Haemaphysalis flava (H. flava) collected from different geographical areas in China,a total of 20 H. flava from Hunan and Henan provinces were used in this study,their internal transcribed spacer Ⅱ (ITS-2) and NADH dehyd ogenase subunit Ⅰ (nad1) sequences were amplified by PCR,and the genetic variations in the obtained sequences were analyzed.Moreover,the phylogenetic relationship of H. flava from two provinces was analyzed,and two phylogenetic trees were established using the obtained ITS-2 and nad1 genes sequence.The results showed that the intra-specific sequence variations of ITS-2 and nad1 genes within H. flava from Hunan and Henan provinces were 0 to 1.9% and 0 to 0.4%,respectively.The intra-specific sequence variations of ITS-2 and nad1 genes within H. flava from Hunan province were 0 to 1.4% and 0 to 0.4%,respectively.The intra-specific sequence variations of ITS-2 and nad1 genes within H. flava from Henan province were 0 to 1.2% and 0 to 0.4%,respectively.The inter-specific sequence variations of ITS-2 and nad1 genes of H. flava were 37.0% to 52.0% and 13.6% to 24.8%,respectively.The phylogenetic analysis showed that all samples are on a clades in two phylogenetic trees established by ITS-2 and nad1 genes sequence.The results indicated that there was genetic variation between H. flava from Hunan and Henan provinces,but the genetic variation and genetic diversity of H. flava from Henan province were higher than that from Hunan province.The phylogenetic relationship of H. flava from Hunan and Henan provinces was closed.The results suggested that there was gene exchange within H. flava from Hunan and Henan provinces,and no genetic differentiation between all samples.

Key words: Haemaphysalis flava; ITS-2 gene; nad1 gene; genetic variation; phylogenetic evolution

CLC Number:

S822

LI Zhongbo, YAO Guomin, LOU Wei, WANG Xiang, CHENG Tianyin, LIU Guohua, HOU Qianghong. Genetic Variation and Phylogenetic Evolution of Haemaphysalis flava from Different Geographical Areas in China[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2150-2159.

References

[1] CHOI C Y,KANG C W,KIM E M,et al.Ticks collected from migratory birds,including a new record of Haemaphysalis formosensis,on Jeju Island,Korea[J].Experimental and Applied Acarology,2014,62(4):557-566.
[2] 李中波.褐黄血蜱、长角血蜱的ITS序列及线粒体基因组研究[D].长沙:湖南农业大学,2018. LI Z B.Study on ITS sequence and the entire mitochondrial genome sequence of Haemaphysalis flava and Haemaphysalis longicornis[D].Changsha:Hunan Agricultural University,2018.(in Chinese)
[3] SHAO R,AOKI Y,MITANI H,et al.The mitochondrial genomes of soft ticks have an arrangement of genes that has remained unchanged for over 400 million years[J].Insect Molecular Biology,2004,13(3):219-224.
[4] LI Z B,CHENG T Y,XU X L,et al.Genetic variation in mitochondrial genes of the tick Haemaphysalis flava collected from wild hedgehogs in China[J].Experimental and Applied Acarology,2017,71(2):131-137.
[5] CHENG W Y,ZHAO G H,JIA Y Q,et al.Characterization of Haemaphysalis flava (Acari:Ixodidae) from Qingling subspecies of Giant Panda (Ailuropoda melanoleuca qinlingensis) in Qinling Mountains (Central China) by morphology and molecular markers[J].PLoS One,2013,8(7):e69793.
[6] XU X L,CHENG T Y,YANG H,et al.De novo sequencing,assembly and analysis of salivary gland transcriptome of Haemaphysalis flava and identification of sialoprotein genes[J].Infection,Genetics and Evolution,2015,32(1):135-142.
[7] SILAGHI C,BECK R,OTEO J A,et al.Neoehrlichiosis:An emerging tick-borne zoonosis caused by Candidatus Neoehrlichia mikurensis[J].Experimental and Applied Acarology,2016,68:279-297.
[8] BENELLI G,PAVELA R,CANALE A,et al.Tick repellents and acaricides of botanical origin:Agreen roadmap to control tick-borne diseases?[J].Parasitology Research,2016,115:2545-2560.
[9] GREAY T L,OSKAM C L,GOFTON A W,et al.A survey of ticks (Acari:Ixodidae) of companion animals in Australia[J].Parasit Vectors,2016,9(1):207.
[10] AKUFFO R,J A M,BRANDFU L,et al.Crimean-Congo hemorrhagic fever virus in livestock ticks and animal handler seroprevalence at an abattoir in Ghana[J].BMC Infectious Diseases,2016,16(1):324-329.
[11] CHINIKAR S.Serological evaluation of Crimean-Congo hemorrhagic fever in humans with high-risk professions living in enzootic regions of Isfahan province of Iran and genetic analysis of circulating strains[J].Vector-Borne Zoonotic Diseases,2012,12(9):733-738.
[12] DUAN D Y,CHENG T Y.Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing[J].Journal of Basic Microbio-logy,2017,57(4):302-308.
[13] CHENG T Y,LI Z B,ZOU A D,et al.PCR-DGGE test for direct identification of intestinal bacterial flora in blood feeding ticks in China[J].Tropical Biomedicine,2016,33(4):663-667.
[14] 和小明,唐欢,程天印.褐黄血蜱唾液菌群结构分析[J].中国预防兽医学报,2016,38(7):588-590. HE X M,TANG H,CHENG T Y.Analysis of the icroflora in the saliva from Haemaphysalis flava[J].Chinese Journal of Animal and Veterinary Sciences,2016,38(7):588-590.(in Chinese)
[15] NIU G,LI J,LIANG M,et al.Severe fever with Thrombocytopenia syndrome virus among domesticated animals,China[J].Emerging Infectious Diseases,2013,19(5):756-763.
[16] LIU L,LIU Y S,LIU G H,et al.Proteomics analysis of faecal proteins in the tick Haemaphysalis flava[J].Parasites Vectors,2018,11(1):89.
[17] YI J N,JIN Y C,LIU J H,et al.Genetic variation in three mitochondrial genes among cattle tick Rhipicephalus microplus originating from four provinces of China[J].Tropical Biomedicine,2019,36(1):1-7.
[18] LI Z B,LIU G H,CHENG T Y.Molecular characterization of hard tick Haemaphysalis longicornis from China by sequences of the internal transcribed spacers of ribosomal DNA[J].Experimental and Applied Acarology,2018,74:171-176.
[19] BHAT S A,SINGH N K,SINGH H,et al.Molecular prevalence of Babesia bigemina in Rhipicephalus microplus ticks infesting cross-bred cattle of Punjab,India[J].Parasite Epidemiology and Control,2017,2:85-90.
[20] 向亮亮.微小扇头蜱、唾液腺、唾液菌群结构的比较[D].长沙:湖南农业大学,2017. XIANG L L.The comparison of microbiome among the whole body,salivary gland and saliva obtained from female adult Rhipicephalus microplus[D].Changsha:Hunan Agricultural University,2017.(in Chinese)
[21] SUN E T,LI C P,NIE L W,et al.The complete mitochondrial genome of the brown leg mite,Aleuroglyphus ovatus (Acari:Sarcoptiformes):Evaluation of largest non-coding region and unique tRNAs[J].Experimental and Applied Acarology,2014,64(2):141-157.
[22] SAITO S,TAMURA K,AOTSUKA T.Replication origin of mitochondrial DNA in insects[J].Genetics,2005,171(4):1695-1705.
[23] SONG S,SHAO R,ATWELL R,et al.Phylogenetic and phylogeographic relationships in Ixodes holocyclus and Ixodes cornuatus(Acari:Ixodidae) inferred from cox1 and ITS-2 sequences[J].International Journal for Parasitology,2011,14(8):871-880.
[24] BUTOONCHOO P,WONGSAWAD C,ROJANAPAIBUL A,et al.Morphology and molecular phylogeny of Raillietina spp.(Cestoda:Cyclophyllidea:Davaineidae) from domestic chicken in Thailand[J].Korean Journal of Parasitology,2016,54:781-790.
[25] LIU G H,GASSER R B,YOUNG N D,et al.Complete mitochondrial genomes of the intermediate form of Fasciola and Fasciola gigantica,and their comparison with F.hepatica[J].Particle and Fibre Toxicology,2014,7(1):150-159.
[26] 李中波,侯强红,尧国民,等.四角赖利绦虫(Raillietina tetragona) ITS-1 rDNA序列的扩增及分析[J].经济动物学报,2020,24(2):107-110. LI Z B,HOU Q H,YAO G M,et al.PCR amplification and sequence analysis of ITS-1 region of Raillietina tetragona[J].Journal of Economic Animal,2020,24(2):107-110.(in Chinese)
[27] GUINDON S,DUFAYARD J F,LEFORT V,et al.New algorithms and methods to estimate maximum-likelihood phylogenies:Assessing the performance of PhyML 3.0[J].Systematic Biology,2010,59:307-321.
[28] BURGER T D,SHAO R,BEATI L,et al.Phylogenetic analysis of ticks (Acari:Ixodida) using mitochondrial genomes and nuclear rRNA genes indicates that the genus Amblyomma is polyphyletic[J].Molecular Phylogenetics and Evolution,2013,64(1):45-50.
[29] LI Z B,FU Y T,CHENG T Y,et al.Mitochondrial gene heterogeneity and population genetics of Haemaphysalis longicornis (Acari:Ixodidae) in China[J].Acta Parasitologica,2019,64(2):360-366.
[30] HAJDUSEK O,SIMA R,AVLLON N,et al.Interaction of the tick immune system with transmitted pathogens[J].Frontiers in Cellular and Infection Microbiology,2013,3:26-41.
[31] ZHOU Y Z,ZHANG H S,CAO J,et al.Epidemiology of toxoplasmosis:Role of the tick Haemaphysalis longicornis[J].Infectious Diseases of Poverty,2016,5(1):14-20.
[32] 温廷桓,陈泽.世界蜱类名1.软蜱与纳蜱科(螨亚纲:蜱目)[J].中国寄生虫学与寄生虫病杂志,2016,34(1):58-69. WENG T H,CHEN Z.The world list of ticks.1.Argasidae and Nuttallielidae (Acari:Ixodida)[J].Chinese Journal of Parasitology and Parasitic Diseases,2016,34(1):58-69.(in Chinese)
[33] ANDOH M,ANDOH R,TERAMOTO K,et al.Survey of Coxiella burnetii in ticks collected from dogs in Japan[J].Journal of Veterinary Medical Science,2013,75(8):1115-1117.
[34] 严芬,程天印.褐黄血蜱的形态学与分子生物学鉴定[J].中国兽医学报,2015,35(6):912-916. YAN F,CHENG T Y.Morphological and molecular identification Haemaphysalis flava[J].Chinese Journal of Veterinary Science,2015,35(6):912-916.(in Chinese)
[35] KULAKOVA N V,KHASNATINOV M A,SIDOROVA E A,et al.Molecular identification and phylogeny of Dermacentor nuttalli (Acari:Ixodidae)[J].Parasitology Research,2014,113:1787-1793.
[36] KANDUMA E G,MWACHARO J M,GITHAKA N W,et al.Analyses of mitochondrial genes reveal two sympatric but genetically divergent lineages of Rhipicephalus appendiculatus in Kenya[J].Parasit Vectors,2016,9:353.
[37] BLOUIN M S.Molecular prospecting for cryptic species of nematodes:Mitochondrial DNA versus internal transcribed spacer[J].International Journal for Parasitology,2002,32:527-531.
[38] YUAN M L,WEI D D,WANG B J,et al.The complete mitochondrial genome of the citrus red mite Panonychuscitri (Acari:Tetranychidae):High genome rearrangement and extremely truncated tRNAs[J].BMC Genomics,2010,11(1):597.
[39] SIMON C,FRATI F,BECKENBACH A,et al.Evolution,weighting,and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers[J].Annals of the Entomological Society of America,1994,87(6):651-701.

Genetic Variation and Phylogenetic Evolution of Haemaphysalis flava from Different Geographical Areas in China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[2]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[3]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[4]	ZHAI Xinguo, ZHENG Peipei, SU Jinhui, LI Qingyang, JIAO Hejing, ZHANG Ruobing, LI Penghui. Genetic Variation and Recombination Analysis of Whole Genome of Porcine Epidemic Diarrhea Virus HB/HEBEU/2020 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3140-3150.
[5]	ZHANG Xinjie, XUE Shaohua, LYU Zixin, HUANG Xirong, CHEN Yuxuan, FAN Kewei, YANG Xiaoyan, DAI Ailing. Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2291-2297.
[6]	YAN Zhong, YANG Hanchun. Surveillance and Genetic Variation Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus in Part Provinces and Municipalities of China from 2018 to 2020 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 424-434.
[7]	TAO Zhiyun, ZHU Chunhong, LIU Hongxiang, SHI Zuhao, ZHANG Shuangjie, XU Wenjuan, SONG Weitao, WANG Zhicheng, LI Huifang. Screening and Identification of Genetic Variation and Functional Genes Related to Egg Production in Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4107-4118.
[8]	XU Yiwei, LI Dan, CHENG Tianyin. Identification of Haemaphysalis flava Egg Proteins and Their Expression Changes During Embryonic Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4410-4419.
[9]	QU Zhehui, ZHANG Xiwen, ZHAO Yu, ZHENG Quanfang, LIAN Huixiang, ZHAO Yunhuan, GUO Xiaoqiu. Genetic Variation Analysis of Major Virulence Genes of Pseudorabies Virus Epidemic Strains in Southern Henan Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3019-3029.
[10]	WANG Tongtong, LIU Mengyuan, ZHANG Jingwen, XI Cankun, JIA Shunan, YU Yue, LIU Wenqiang, REN Huiying, WANG Changfa, LI Liangliang. Isolation,Identification and Genetic Characterization of Equine Herpesvirus Type 8 from Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3030-3037.
[11]	XIAO Yi, WANG Mengwen, ZHANG Xiuying, GULZAR Abdukerim, HAN Jianlin, WANG Yutao. Genetic Diversity,Origin and Evolution Analysis of Ovis ammon polii Based on mtDNA Cytb Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1681-1690.
[12]	LI Zhongbo, YAO Guomin, HOU Qianghong, CHENG Tianyin. Proteomic Profiling of the Midgut Contents of Female Haemaphysalis flava at Different Digestion Stage [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 607-618.
[13]	XU Hu, PAN Yan, SONG Mingxin. Genomic Characteristics of 2.1d Subtype Epidemic Strain of Classical Swine Fever in China in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2561-2570.
[14]	ZHANG Xiyi, WEN Guilan, GUAN Guodan, CHEN Guang, TIAN Lang, YANG Baiqi. Cloning and Genetic Variation Analysis of NSP2 Gene of Porcine Reproductive and Respiratory Syndrome Virus Guizhou Strain GZ-R [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3659-3666.
[15]	JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017 [J]. , 2019, 46(5): 1474-1482.