褐黄血蜱卵蛋白的鉴定及在卵孵化过程中的量变

doi:10.16431/j.cnki.1671-7236.2022.11.032

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the protein components of Haemaphysalis flava egg and their roles in embryonic development, so as to lay a foundation for the screening vaccine antigen of anti-tick reproduction.【Method】 The egg proteins of Haemaphysalis flava incubated for 0, 7, 14 and 21 days were extracted and digested by the FASP method.The unique peptides were separated and detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.The midgut transcriptome library of Haemaphysalis flava was searched to identify proteins and annotated with NCBI.The iBAQ and LFQ of each protein were calculated by Maxquant, and the abundance of each protein at different incubation time was analyzed.【Result】 1 044 unique peptides were detected and 291 proteins were identified, 112 of which were high confidence (unique peptides ≥ 2), only 39 existing relevant literatures. The 39 proteins had been studied belong to six groups, including enzyme, protease inhibitor, transporter, heat shock protein, cytoskeleton protein, and unknown protein.Enzymes, protease inhibitors, transporters, and heat shock proteins all coexisted with family proteins.Quantitative analysis showed that the highest abundance was Contig14782, followed by Contig6575 in fresh eggs of Haemaphysalis flava.By the end of incubation, among the 20 highly abundant proteins, only Contig2242 was significantly increased (P<0.05), 14 were significantly decreased (P<0.05), and 5 were not significantly changed.GO functional enrichment analysis showed that the high confidence proteins identified in this work were mainly localized in the extracellular space, which was involved in immunoregulatory processes, and the molecular functions were correlated with protein binding, and RNA binding.【Conclusion】 Haemaphysalis flava egg protein composition was complex and numerous, including enzyme, protease inhibitor, transporter, heat shock protein, cytoskeleton protein, and unknown protein, of which enzyme was the most one.During incubation, most enzymes and protease inhibitors decreased significantly.

Key words: Haemaphysalis flava; egg proteins; LC-MS/MS; quantitative analysis

CLC Number:

S852.74^＋6

XU Yiwei, LI Dan, CHENG Tianyin. Identification of Haemaphysalis flava Egg Proteins and Their Expression Changes During Embryonic Development[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4410-4419.

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Identification of Haemaphysalis flava Egg Proteins and Their Expression Changes During Embryonic Development

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