闽粤地区PPV7与PCV2的混合感染调查及PPV7 Cap基因的遗传变异分析

doi:10.16431/j.cnki.1671-7236.2022.06.029

Abstract

Abstract: 【Objective】 To investigate the co-infection situation of Porcine parvovirus (PPV7) and Porcine circovirus type 2 (PCV2)in Fujian and Guangdong, and to understand the molecular genetic characteristics of PPV7 Cap gene.【Method】 The blood sample of 432 infected pigs from 69 pig farms in Fujian and Guangdong were collected to detect PPV7 and PCV2 by PCR.The PPV7 Cap gene of positive samples was cloned and sequenced.The DNAStar software was used to analyze the nucleotide and amino acid sequences of PPV7 Cap gene, and Mega 7.0 software was used to draw the genetic evolution tree.【Result】 The results showed that the positive rate of PPV7 was 21.99% (96/432), the positive rate of farms was 53.62%(37/69), and the positive rate of PCV2 was 54.17% (234/432), and the co-infection rate of PCV2 and PPV7 was 13.43% (58/432).The 17 PPV7 Cap gene sequences were amplified using PCR.Nucleotide homology analysis revealed that the homology of the 17 PPV7 Cap gene sequences was 85.6%-100%, and the homology with reference strains was 85.8%-99.0%.Amino acid sequence comparison analysis revealed that the amino acid homology of the 17 PPV7 Cap protein sequences was 87.6%-100%, and the amino acid homology with reference strains was 82.6%-98.7%.Phylogenetic analysis of Cap gene showed that PPV7 could be divided into five main evolutionary branches of PPV7a-PPV7e, among which 9 isolates belonged to PPV7a subtype, 3 isolates belonged to PPV7b subtype, 4 isolates belong to PPV7c subtype, and only 1 isolates isolates belonged to PPV7e subtype.【Conclusion】 This study indicated that PPV7 was widely prevalent in Fujian and Guangdong regions, and had a high co-infection rate with PCV2, which might be the pathogenic factor of Porcine circovirus associated disease(PCVAD).The genetic diversity of PPV7 isolates was abudant in both regions, and PPV7a was the dominant strain at present.The findings of this study provided theoretical basis and data reference for PPV7 prevention and control and vaccine research.

Key words: Porcine parvovirus type 7(PPV7); Cap gene; genetic variation; homology analysis

CLC Number:

S852.65

ZHANG Xinjie, XUE Shaohua, LYU Zixin, HUANG Xirong, CHEN Yuxuan, FAN Kewei, YANG Xiaoyan, DAI Ailing. Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2291-2297.

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Investigation of Co-infection of PPV7 and PCV2 and Genetic Variation Analysis for PPV7 Cap Gene in Fujian and Guangdong

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