重庆地区犬圆环病毒检测及序列分析

doi:10.16431/j.cnki.1671-7236.2020.10.004

Abstract

Abstract: The aim of this study was to investigate the prevalence of canine circovirus (CCV) in Chongqing in recent years,and to explore the characteristics of the field strains in Chongqing.In this study,100 dog serum samples collected in Chongqing in 2017 were tested for CCV nucleic acid by PCR,and the obtained CCV positive samples were amplified and sequenced with the full length genome,and the Chongqing CCV strain isolated was analyzed by MegAlign,Mega 6.0 and RDP4 software.Four positive sample were found,with a positive rate of 4%,and three full length CCV genomes (CQ76,CQ79,and CQ82) were obtained,with a total length of 2 062 nt.Compared with the previously reported genome of CCV with a length of 2 063 nt,one base was missing,which was located downstream of the stem ring structure in the 5'-intergenic region.The length of the 5'-intergenic region and 3'-intergenic region between the two ORFs of the three strains were 134 (1 929-2 062 nt) and 203 nt (913-1 115 nt),respectively.The homology of CQ76,CQ79,and CQ82 ranged from 99.8% to 100%,among which,CQ76 and CQ82 only had synonymous mutations at the 2 019 locus.The homology of genome sequences of the three Chongqing strains and other strains was 82.7% to 97.1%,among which,the degree of variation of ORF2 was greater than that of ORF1.Based on virus ORF2 sequences and genome-wide respectively build Neighbor-Joining in the evolutionary tree,CQ76,CQ79 and CQ82 all belonged to the same subgroup,which was close to the Chinese strain 204,which belonged to genotype Ⅱ,and the homology was 96.5% to 96.7%.RDP4 recombinant analysis indicated that the genomes of CQ76,CQ79 and CQ82 strains were all the recombinant sequences of 204 and 388 strains from Guangxi,and the recombinant region was located in ORF2.Above results enriched the epidemiological and genetic evolution information of CCV and provided further basic data for prevention and control research.

Key words: canine circovirus (CCV); sequence analysis; homology analysis

CLC Number:

S852.65

YAN Xiukui, HAN Zhixiao, DU Qian, WANG Wei, ZHU Yuanzhi, XIN Jialiang, YI Chizhe, BI Jingshan, SUN Wenchao, ZHENG Min. Detection and Sequence Analysis of Canine Circovirus in Chongqing[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3069-3077.

References 22

[1]	KAPOOR A,DUBOVI E J,HENRIQUEZ-RIVERA J A,et al.Complete genome sequence of the first canine circovirus[J].Journal of Virology,2012,86(12):7018.
[2]	殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997. YIN Z,LIU J H.Animal Virology[M].11th Edition.Beijing:Science Press,1997.(in Chinese)
[3]	ANDERSON A,HARTMANN K,LEUTENEGGER C M,et al.Role of canine circovirus in dogs with acute haemorrhagic diarrhoea[J].Veterinary Record,2017,180(22):542.
[4]	HSU H S,LIN T H,WU H Y,et al.High detection rate of dog circovirus in diarrheal dogs[J].BMC Veterinary Research,2016,12(1):116.
[5]	GIULIA D O,ELEONORA L,NICOLA D,et al.A molecular survey for selected viral enteropathogens revealed a limited role of canine circovirus in the development of canine acute gastroenteritis[J].Veterinary Microbiology,2017,204:54-58.
[6]	HOGEZAND R A V,BANFFER D,ZWINDERMAN A H,et al.Antigenic analysis of canine parvovirus strains isolated in Italy[J].New Microbiologica,2000,23(1):93-96.
[7]	PIEWBANG C,JO W K,PUFF C,et al.Novel canine circovirus strains from Thailand:Evidence for genetic recombination[J].Scientific Reports,2018,8(1):7524-7531.
[8]	韩知晓,杜倩,汪伟,等.犬圆环病毒Rep基因SYBR GreenⅠ Real-time PCR方法的建立及应用[J].中国兽医科学,2019,49(12):1520-1526. HAN Z X,DU Q,WANG W,et al.Establishment and application of SYBR Green Ⅰ Real-time PCR for canine circovirus Rep gene[J].Veterinary Science in China,2019,49(12):1520-1526.(in Chinese)
[9]	孙文超,曹慧慧,郑敏,等.犬圆环病毒全基因组克隆与序列分析[J].病毒学报,2016,32(4):429-435. SUN W C,CAO H H,ZHENG M,et al.Canine circovirus genome cloning and sequence analysis[J].Chinese Journal of Virology,2016,32(4):429-435.(in Chinese)
[10]	孙文超,解长占,赵冠宇,等.犬圆环病毒实时荧光定量PCR检测方法的建立[J].动物医学进展,2017,38(5):6-10. SUN W C,XIE C Z,ZHAO G Y,et al.Development of a Real-time PCR method for detection of dog circovirus[J].Progress in Veterinary Medicine,2017,38(5):6-10.(in Chinese)
[11]	曹亮,孙文超,鲁会军,等.犬圆环病毒及犬细小病毒双重PCR检测方法的建立[J].中国兽医科学,2017,47(9):1140-1144. CAO L,SUN W C,LU H J,et al.Establishment of duplex PCR for the detection of canine circovirus and canine parvovirus[J].Chinese Veterinary Science,2017,47(9):1140-1144.(in Chinese)
[12]	孙文超,汪伟,曹亮,等.犬圆环病毒ORF3基因的克隆及表达载体的构建[J].中国病原生物学杂志,2018,13(2):109-111. SUN W C,WANG W,CAO L,et al.Cloning of canine circovirus ORF3 and construction of an expression vector[J].Journal of Pathogen Biology,2018,13(2):109-111.(in Chinese)
[13]	汪伟,辛佳亮,杜倩,等.犬圆环病毒Cap蛋白的生物信息学分析及原核表达[J].中国动物传染病学报,2020,28(2):86-91. WANG W,XIN J L,DU Q,et al.Bioinformatics analysis and prokaryotic expression of canine circovirus Cap protein[J].Chinese Journal of Animal Infectious Diseases,2020,28(2):86-91.(in Chinese)
[14]	NIU L D,WANG Z,ZHAO L L,et al.Detection and molecular characterization of canine circovirus circulating in Northeastern China during 2014-2016[J].Archives of Virology,2020,165(1):137-143.
[15]	DECARO N,MARTELLA V,DESARIO C,et al.Genomic characterization of a circovirus associated with fatal hemorrhagic enteritis in dog,Italy[J].PLoS One,2014,9(8):e105909.
[16]	PLOG S,KUBISKI S,ENDERS J,et al.Detection of dog circovirus in a dog without typical lesions-what do we know about subclinical infection?[J].Journal of Comparative Pathology,2017,156(1):75.
[17]	LI L,MCGRAW S,ZHU K,et al.Circovirus in tissues of dogs with vasculitis and hemorrhage[J].Emerging Infectious Diseases,2013,19(4):534-541.
[18]	KOKILA P A,BALAGANGATHARATHILAGAR M,CHANDRASEKARAN D,et al.Prevalence of enteropathogens and their antibiotic sensitivity pattern in puppies with hemorrhagic gastroenteritis[J].Veterinary World,2017,10(8):859-863.
[19]	KOTSIAS F,BUCAFUSCO D,NUNEZ D A,et al.Genomic characterization of canine circovirus associated with fatal disease in dogs in South America[J].PLoS One,2019,14(6):e0218735.
[20]	LI L,KAPOOR A,SLIKAS B,et al.Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces[J].Journal of Virology,2010,84(4):1674-1682.
[21]	TODD D.Avian circovirus diseases:Lessons for the study of PMWS[J].Veterinary Microbiology,2004,98(2):169-174.
[22]	严楠,冯帆,李明翔,等.四川部分地区犬圆环病毒的病原检测及基因组特征[J].中国预防兽医学报,2019,41(3):313-316. YAN N,FENG F,LI M X,et al.Detection and genome characteristics of canine circovirus in part areas of Sichuan[J].Chinese Journal of Preventive Veterinary Medicine,2019,41(3):313-316.(in Chinese)

Detection and Sequence Analysis of Canine Circovirus in Chongqing

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