基于mtDNA D-loop序列探究门源白牦牛与天祝白牦牛间的遗传差异

doi:10.16431/j.cnki.1671-7236.2021.05.015

Abstract

Abstract: In order to explore the maternal genetic difference between Menyuan White yaks in Qinghai province and Tianzhu White yaks in Gansu province,the mtDNA D-loop sequences of 31 Menyuan White yaks were sequenced in this study.Combined with 235 D-loop sequences of Tianzhu White yaks reported in GenBank,266 White yaks D-loop sequences were analyzed.The results showed that 80 polymorphic sites were detected in the 638 bp D-loop sequences,including 5 single polymorphic sites and 75 parsimony informative sites after excluding 64 InDel sites.A total of 56 haplotypes were identified according to the nucleotide sequence variations.Totally,Menyuan White yaks had 7 specific haplotypes and Tianzhu White yaks owned 43 specific haplotypes,but only 2 haplotypes were shared by them.The haplotypes of Menyuan White yaks belonged to A,B and C haplogroups,while the haplotypes of Tianzhu White yaks falled within A,B,C,D,E and F haplogroups and contained 4 Bos taurus haplotypes,which indicated that there was genetic introgression of Bos taurus in Tianzhu White yak breed.The haplotype diversity and nucleotide diversity of Menyuan White yaks were 0.862 and 0.007,respectively,while those of Tianzhu White yaks were 0.946 and 0.014,respectively,which showed that the level of genetic diversity of Tianzhu White yaks was higher than that of Menyuan White yaks.The genetic differentiation index (Fst) between two populations was 0.114,which indicated that the genetic differentiation between them was moderate.The result of phylogenetic analysis showed that the 56 haplotypes could be divided into two distinct lineages (Ⅰ and Ⅱ),indicating that White yaks had two maternal origins.In conclusion,both Menyuan White yaks and Tianzhu White yaks had rich maternal genetic diversity,but the latter had higher level of genetic diversity than the former.There was a moderate differentiation between two populations and Tianzhu White yaks had a certain degree of Bos taurus genetic introgression.Each White yak breed (population) was composed of two maternal lineages,suggesting that White yaks had two maternal origins.Considering the moderate genetic differentiation between two populations and the specific haplotypes owned by them,we suggested that they should be considered as independent "genetic unit"for genetic resource protection and then corresponding conservation and breeding practice would be carried out.

Key words: White yaks; mtDNA; D-loop region; genetic diversity; differentiation; maternal origin

CLC Number:

S823.85

LI Guangzhen, MA Zhijie, CHEN Shengmei, GA Buzang, LI Ruizhe. Genetic Difference Between Menyuan White Yaks and Tianzhu White Yaks Based on mtDNA D-loop Sequences[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1647-1654.

References

[1] WIENER G,HAN J L,LONG R J.The Yak[M].Bangkok:Food and Agriculture Organization of the United Nations Regional Office for Asia and the Pacific,2003.
[2] 钟金城,赵素君,陈智华,等.牦牛品种的遗传多样性及其分类研究[J].中国农业科学,2006,39(2):389-397. ZHONG J C,ZHAO S J,CHEN Z H,et al.Study on genetic diversity and classification of the yak[J].Scientia Agricultura Sinica,2006,39(2):389-397.(in Chinese)
[3] 马凤莲,李黎,王文福,等.互助白牦牛8项血液生化指标测定[J].青海畜牧兽医杂志,1996,26(2):18-19. MA F L,LI L,WANG W F,et al.Determination of 8 blood physiological indexes in Huzhu White yak[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,1996,26(2):18-19.(in Chinese)
[4] BRUFORD M W,BRADLEY D G,LUIKART G,et al.DNA markers reveal the complexity of liverstock domestication[J].Nature Reviews Genetics,2003,4(11):900-910.
[5] JACKSON C B,ZBINDEN C,GALLATI S,et al.Heterologous expression from the human D-loop in organelle[J].Mitochondrion,2014,17:67-75.
[6] SRIVASTAVA G K,RAJPUT N,JADAV K K,et al.Single nucleotide markers of D-loop for identification of Indian wild pig (Sus scrofa cristatus)[J].Veterinary World,2015,8(4):532-536.
[7] MAITSEV A N,STAKHEEV V V,BOGDANOV A S,et al.Phylogenetic relationships of intraspecific forms of the house mouse Mus musculus:Analysis of variability of the control region (D-loop) of mitochondrial DNA[J].Doklady Biological Sciences,2015,465(1):285-288.
[8] 马志杰.牦牛线粒体基因组研究进展[J].中国农业大学学报,2014,19(3):154-161. MA Z J.Research progress of the yak (Bos grunniens) mitochondrial genome[J].Journal of China Agricultural University,2014,19(3):154-161.(in Chinese)
[9] 赖松家,王玲,刘益平,等.中国部分牦牛品种线粒体DNA遗传多态性研究[J].遗传学报,2005,32(5):463-470. LAI S J,WANG L,LIU Y P,et al.Study on mitochondrial DNA genetic polymorphism of some yak breeds in China[J].Acta Genetica Sinica,2005,32(5):463-470.(in Chinese)
[10] GUO S C,SAVOLAINEN P,SU J P,et al.Origin of mitochondrial DNA diversity of domestic yaks[J].BMC Evolutionary Biology,2006,6:73.
[11] 郭松长,祁得林,陈桂华,等.家牦牛线粒体DNA(mtDNA)遗传多样性及其分类[J].生态学报,2008,28(9):4286-4294. GUO S C,QI D L,CHEN G H,et al.Genetic diversity and classification of domestic yaks inferred from mitochondrial DNA sequences[J].Acta Ecologica Sinica,2008,28(9):4286-4294.(in Chinese)
[12] WANG Z F,SHEN X,LIU B,et al.Phylogeo-graphical analyses of domestic and wild yaks based on mitochondrial DNA:New data and reappraisal[J].Journal of Biogeography,2010,37(12):2332-2344.
[13] 成述儒,曾玉峰,王欣荣,等.甘肃境内6个牦牛群体mtDNA D-环序列遗传多样性与聚类分析[J].华北农学报,2014,29(3):16-21. CHEN S R,ZENG Y F,WANG X R,et al.Genetic diversity and cluster analysis of six yak populations in Gansu province using mitochondrial D-loop sequence[J].Acta Agriculturae Boreali-Sinica,2014,29(3):16-21.(in Chinese)
[14] YUE X P,LIANG Y S,LIANG Y L,et al.Comprehensive investigation of nucleotide diverdity in yaks[J].Animal Genetics,2016,47(6):752-755.
[15] 马志杰,钟金城,韩建林,等.野牦牛(Bos grunniens mutus) mtDNA D-loop区的遗传多样性[J].生态学报,2009,29(9):4798-4803. MA Z J,ZHONG J C,HAN J L,et al.Genetic diversity in the mtDNA D-loop region of wild yak (Bos grunniens mutus)[J].Acta Ecologica Sinica,2009,29(9):4798-4803.(in Chinese)
[16] MA Z J,ZHONG J C,HAN J L,et al.Genetic diversity and demographic history of wild yak (Bos grunniens mutus) inferred from mtDNA D-loop sequences[J].African Journal of Biotechnology,2010,9(46):7805-7810.
[17] 侯孟典,宋仁德,王会,等.玉树牦牛与四个牦牛群体线粒体DNA D-loop区遗传多样性分析[J].家畜生态学报,2020,41(3):21-26. HOU M D,SONG R D,WANG H,et al.Analysis on genetic diversity of mtDNA D-loop region from Yushu yak population and other four yak population[J].Journal of Domestic Animal Ecology,2020,41(3):21-26.(in Chinese)
[18] 李广祯,马志杰,陈生梅,等.青海省曲麻莱牦牛的母系遗传多样性及遗传背景探究[J].青海大学学报,2020,38(1):27-31. LI G Z,MA Z J,CHEN S M,et al.Maternal genetic diversity and genetic background of Qumalai yaks in Qinghai province[J].Journal of Qinghai University,2020,38(1):27-31.(in Chinese)
[19] 李瑞哲,马志杰,陈生梅,等.基于mtDNA D-loop序列分析探究青海雪多牦牛的遗传多样性及母系遗传背景[J].青海畜牧兽医杂志,2018,48(2):1-3. LI R Z,MA Z J,CHEN S M,et al.Exploration on genetic diversity and matrilineal genetic background of Xueduo yak based on mtDNA D-loop sequence analysis[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,2018,48(2):1-3.(in Chinese)
[20] 李静,郭丽君,王莉,等.喀喇昆仑-帕米尔地区牦牛mtDNA D-loop区遗传多样性及系统发育分析[J].中国畜牧兽医,2020,47(8):2481-2492. LI J,GUO L J,WANG L,et al.Analyses of genetic diversity and phylogeny of mtDNA D-loop from yak in Karakoram-Pamir area[J].China Animal Husbandry & Veterinary Medicine,2020,47(8):2481-2492.(in Chinese)
[21] 胡丹,钟金城,柴志欣.新疆塔什库尔干牦牛mtDNA Cytb基因和D-loop区遗传多样性及系统进化分析[J].家畜生态学报,2018,39(11):11-16. HU D,ZHONG J C,CHAI Z X.Genetic diversity and phyletic evolution on mtDNA Cytb gene and D-loop region of Taxkorgan yak[J].Journal of Domestic Animal Ecology,2018,39(11):11-16.(in Chinese)
[22] 张成福,徐利娟,姬秋梅,等.西藏牦牛mtDNA D-loop区的遗传多样性及其遗传分化[J].生态学报,2012,32(5):1387-1395. ZHANG C F,XU L J,JI Q M,et al.Genetic diversity and evolution relationship on mtDNA D-loop in Tibetan yaks[J].Acta Ecologica Sinica,2012,32(5):1387-1395.(in Chinese)
[23] WRIGHT S.Evolution and the Genetics of Populations vol.4:Variability Within and Among Natural Popula-tions[M].Chicago:University of Chicago Press,1978.
[24] CHEN N B,CAI Y D,CHEN Q M,et al.Whole-genome resequencing reveals world-wide ancestry and adaptive introgression events of domesticated cattle in East Asia[J].Nature Communications,2018,9(1):2337.
[25] QI X B,HAN J L,WANG G,et al.Assessment of cattle genetic introgression into domestic yak popula-tions using mitochondrial and microsatellite DNA markers[J].Animal Genetics,2010,41(3):242-252.

Genetic Difference Between Menyuan White Yaks and Tianzhu White Yaks Based on mtDNA D-loop Sequences

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MENG Chaoqun, LI Chengping, ZHAO Wei, QIN Xuyong, ZHOU Guoli. Effect of miR-137-3p on Adipogenic Differentiation of Preadipocytes 3T3-L1 by Targeting MAX Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1928-1937.
[2]	LI Zhongbo, HOU Qianghong, DUAN Deyong. Haplotypes Diversity,Genetic Differentiation and Phylogenetic Analysis of Aedes albopictus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1452-1460.
[3]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[4]	ZHEN Zhen, WANG Mei, WANG Yimin, HU Debao, ZHANG Linlin, LI Xin, GUO Yiwen, GUO Hong, DING Xiangbin. Effect of RNA Methylation Transfer Enzyme METTL3 on the Proliferation and Myogenic Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1025-1036.
[5]	TAN Haoyun, LIU Qian, HU Debao, ZHANG Linlin, LI Xin, DING Xiangbin, GUO Hong, GUO Yiwen. Effects of Interference lnc721 on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3292-3300.
[6]	GAO Yefan, SONG Hanan, WANG Yunan, WU Yue, NIU Ruili, ZONG Xianchun, GUAN Weijun. Study on the Isolation, Culture and Differentiation Potential of Limbal Stem Cells from Beijing You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3372-3381.
[7]	WANG Nan, FENG Baoliang, ZHENG Yunxi, HUANG Lei, WANG Yue, XU Songsong, ZHANG Xiuling, LIU Zhiguo, LI Kui, MU Yulian. Effects of WIP1 Gene on Proliferation and Differentiation of 3T3-L1 Preadipocytes and Its Expression in Different Growth Stages of Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2869-2879.
[8]	ZHANG Xueping, SHI Bingang, JIN Xiayang, WANG Xiangyan, LAN Lijuan, SHI Yu, QI Youpeng, ZHAO Shijie, LI Shaobin, HU Jiang. Expression Characterization Analysis of KLF7 Gene and Effects of Its Overexpression on Proliferation and Differentiation of Preadipocytes in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2920-2930.
[9]	XIONG Chenyong, YIN Yanwen, SHI Kaichuang, LI Jun, ZHENG Min, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, ZHOU Hongjin, HUANG Hailian, XIE Shouyu, LI Zongqiang. Genetic Diversity Analysis of Duck Tembusu Virus in Guangxi During 2019-2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3099-3111.
[10]	PENG Caichun, LI Binqiang, WANG Yeying, SU Haijun. Genetic Diversity Analysis of Sus scrofa Population in Guizhou Based on Mitochondrial CytbGene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2601-2612.
[11]	YUAN Jiahui, ZHANG Pengxiang, JI Shusen, LU Jiayin, LUO Xiaomao, YAN Yi, WANG Haidong. Mechanism of miR-140-5p on Adipogenic Differentiation of Preadipocytes 3T3-L1 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2631-2644.
[12]	LYU Xiangzhou, ZHANG Qi, LI Xin, YU Yongsheng. Effect of Puerarin on Adipogenic Differentiation of Porcine Preadipocytes Based on AMPK Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2088-2097.
[13]	ZHANG Qiang, LUOSANG Dunzhu, BASANG Wangdui, PENG Yangyang, E Guangxin, NIMA Ciren, SUOLANG Duoji, BA Duo, DAN Ba, XIAN Lili, DANBA Jiacan, ZHI Zhang, PINGCUO Zhandui. Estimation of Genetic Diversity and Population Structure of Five Tibet Yak Populations Using Microsatellites Markers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2228-2238.
[14]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[15]	YANG Lili, ZHANG Qiongwen, ZHANG Yu, LIU Xiaoxiao, CHEN Ting, JIANG Mingsheng. Effect of miR-495-3p on Proliferation and Differentiation of Goat Skeletal Muscle Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1271-1279.