牦牛EPO、PPARα基因SNP与高原低氧适应的相关性分析

doi:10.16431/j.cnki.1671-7236.2021.05.014

Abstract

Abstract: The aim of this study was to explore the correlation between genetic polymorphism of erythropoietin (EPO), peroxisome proliferator-activated receptor α (PPARα) genes and hypoxia adaptability in yak.375 ears sample from 6 yak groups (Zhongdian yak,Maiwa yak,Sibu yak,Leiwuqi yak,Pali yak and Shenzha yak) and Sanjiang cattle at different altitudes were collected to extracted DNA and constructed DNA pool.The polymorphism of EPO and PPARα genes were detected by direct sequencing and PCR-RFLP.Finally,the association between the SNPs of the candidate genes and the high altitude hypoxia adaptation of yak were analyzed by SHEsis.The results showed that both EPO and PPARα genes had 3 SNPs sites,in EPO gene there were rs527G→A,rs1031A→T and rs1192T→C,while in PPARα gene the 3 SNPs sites were rs77363C→T,rs77471C→A and rs77534C→T.The fitness test indicated that the three SNPs sites of EPO gene all conformed to Hardy-Weinberg equilibrium;The rs77363C→T locus of PPARα gene was accorded with Hardy-Weinberg equilibrium in 6 yak groups (P>0.05).However,rs77471C→A and rs77534C→T of PPARα gene were deviated in Maiwa yak (P<0.05).The haplotype analysis showed that the distribution frequency of the ATC haplotype of EPO gene increased in high altitude yaks with the increase of altitude.Furthermore,the frequency of the TAC haplotype of PPARα gene was significantly higher than other haplotypes in 6 yak groups.Taken together,EPO and PPARα genes could be used as molecular markers for further exploring the mechanism of high altitude hypoxia adaptation of yak.

Key words: yak; EPO gene; PPARα gene; SNP; high altitude hypoxia adaptability

CLC Number:

S823.85

ZHAO Liling, XIANG Chao, WANG Hui, ZHONG Jincheng, CHAI Zhixin, WANG Jikun, WANG Jiabo, WU Zhijuan, XIN Jingwei, JI Qiumei. The Correlation Analysis Between EPO,PPARα genes SNP and High Altitude Hypoxia Adaptation in Yak[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1633-1646.

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The Correlation Analysis Between EPO,PPARα genes SNP and High Altitude Hypoxia Adaptation in Yak

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