China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (4): 1452-1460.doi: 10.16431/j.cnki.1671-7236.2023.04.017

• Genetics and Breeding • Previous Articles     Next Articles

Haplotypes Diversity,Genetic Differentiation and Phylogenetic Analysis of Aedes albopictus

LI Zhongbo1,2, HOU Qianghong1, DUAN Deyong2   

  1. 1. HuaiHua Vocational and Technical College, Huaihua 418000, China;
    2. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
  • Online:2023-04-05 Published:2023-04-06

Abstract: 【Objective】 The purpose of this study was to explore the haplotypes diversity,genetic differentiation and phylogenetic relationships of Aedes albopictus in different populations in Huaihua district,Hunan province.【Method】 Thirty Aedes albopictus were collected from Huaihua district,Hunan province.The partial sequences of the ribosomal 18S rRNA and cytochrome c oxidase subunit 1 (cox1) gene from Aedes albopictus were amplified using PCR method.The variation sites on both genes sequences were analyzed with ClustalX and the haplotypes diversity,genetic differentiation and phylogenetic relationships of Aedes albopictus were studied by Dnasp 5.0,Network 4.6,Arlequin version 3.0,PhyML 3.0 and Mega 5.0,respectively.【Result】 The results showed that the 18S rRNA and cox1 gene sequences of all samples was 489 and 1 004 bp,respcetively.A total of 30 mutation loci were found in the 18S rRNA sequences of Aedes albopictus,including 17 haplotypes (A1 to A17,Hd=0.9149),among which haplotype A5 was the oldest haplotype.The gene differentiation coefficient (Gst),genetic differentiation coefficient (Fst) and gene flow (Nm) of 18S rRNA gene of Aedes albopictus were 0.05614,0.24001 and 0.792,respectively.There were 2 mutation loci and 3 haplotypes (B1 to b3,Hd=0.1908) in the cox1 gene sequence of Aedes albopictus.Haplotype B1 was the oldest haplotype.The Gst,Fst and Nm of cox1 gene of Aedes albopictus were 0.05000,0.04762 and 4.999,respectively.Among the phylogenetic tree,30 Aedes albopictus from Huaihua were located in a branch of two developmental trees.【Conclusion】 The results indicated that there was a certain degree of genetic variation and haplotype polymorphism among the individuals and populations of Aedes albopictus from Huaihua district,Hunan province.However,the haplotypes and individuals had the close genetic relationships,and the frequent genetic exchanges occurred between the individuals of the two populations,whithout genetic differentiation.

Key words: Aedes albopictus; haplotypes diversity; gene flow; genetic differentiation; phylogenetic analysis

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