猪圆环病毒2型天津及周边地区分离株的遗传演化分析

doi:10.16431/j.cnki.1671-7236.2018.05.026

Abstract

Abstract:

To investigate the genetic variation of porcine circovirus type 2 (PCV2) in Tianjin and its surrounding areas,PCV2 whole genes and ORF2 genes of 24 PCV2 strains from the infected pigs in different regions were cloned,sequenced,and nucleotide sequence homology and genetic evolution were analyzed.The results showed that 24 strains had the full length of 1 767 or 1 768 bp,and the homology of complete genomic nucleotides among 24 strains and reference strains on NCBI was from 93.6% to 99.8%,and the homology of 24 strains was from 94.0% to 99.9%.At the same time,the homology of ORF2 gene among 24 strains and reference strains on NCBI was from 87.3% to 99.3%,and the homology of 24 strains was from 89.0% to 100.0%,and the sequence homology of three isolates of 10084F4,R14040 and R14086 was 100.0%.Phylogenetic analysis showed that the PCV2 strains could be classified into two groups,among which 23 strains belonged to PCV2b,1 strain belonged to PCV2a,which showed that PCV2b group strain was predominant prevailing strain in Tianjin and its surrounding areas,this provided some theory for the study of prevalence and variation of PCV2.

Key words: porcine circovirus type 2 (PCV2); cloning; phylogenetic analysis

CLC Number:

S852.65

WANG Lili, ZHENG Li, LU Chao, REN Weike, ZHANG Li, LI Fuqiang, YAN Minghua. Phylogenetic Analysis of Porcine Circovirus Type 2 Isolated from Tianjin and Its Surrounding Areas[J]. , 2018, 45(5): 1331-1340.

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Phylogenetic Analysis of Porcine Circovirus Type 2 Isolated from Tianjin and Its Surrounding Areas

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