南宁地区犬细小病毒VP2基因的克隆及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2018.12.005

Abstract

Abstract:

In order to investigate the prevalence and mutation of canine parvovirus (CPV) in Nanning,Guangxi,this study extracted the viral genomic DNA of 12 positive samples that were initially diagnosed as CPV.Using the extracted samples DNA as templates,the entire VP2 gene sequence was amplified and sequenced.In this experiment,the VP2 gene of all 12 positive strains were compared with the VP2 gene of 17 domestic and foreign CPV isolates registered in GenBank.The results showed that the VP2 gene fragments of 12 strains were successfully amplified with the size about 1 755 bp,and the homology between VP2 gene of 12 positive strains was between 99.2% and 100.0%,among which NN01 and NN07,NN02 and NN06 were the highest,accounting for 100.0%.The homology of VP2 gene between the positive sample strain and other domestic isolates was 97.6% to 100.0%,among which NN08,NN10 and NN04 were the highest with CPV-ZJ1579,all of which were 100.0%,belonging to CPV-2a subtype.The homology between positive sample strains and foreign representative strains was 98.1% to 99.8%.Phylogenetic tree analysis showed that among the 12 strains,3 strains were CPV-2a subtype,3 strains were CPV-2b subtype,and 6 strains were CPV-2c subtype.This was the first time that CPV-2c subtype CPV had been found in Nanning area since the isolation of CPV-2c subtype CPV in Guangxi in early 2018,which indicated that the epidemic of CPV-2c subtype CPV was slowly increasing in China.This experiment indicated that CPV-2a,CPV-2b and CPV-2c coexisted in Nanning area of Guangxi,but the proportion of CPV-2c subtype was larger than that of other areas,which also provided new prevention and control information for this area.In addition to paying attention to the traditional epidemic subtypes such as CPV-2a and CPV-2b,the prevention and control of CPV-2c subtype CPV should be paid more attention to in the actual prevention and control work of CPV.

Key words: canine parvovirus (CPV); CPV-2c; VP2 gene; phylogenetic analysis

CLC Number:

S852.69⁺9.2

XU Run, HUANG Huaxu, XU Xiaoming, LI Zhengtai, MA Lin, LAO Xiaoxiang, WU Xianshi. Cloning and Phylogenetic Analysis of VP2 Gene of Canine Parvovirus in Nanning Region[J]. , 2018, 45(12): 3346-3354.

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Cloning and Phylogenetic Analysis of VP2 Gene of Canine Parvovirus in Nanning Region

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