犬细小病毒CPV-BJ03/17株分离鉴定及VP2基因遗传进化分析

doi:10.16431/j.cnki.1671-7236.2018.07.026

Abstract

Abstract:

In order to study the genetic variation of current domestic canine parvovirus (CPV),the intestinal tissue were collected from a suspected CPV infected canine in Beijing.After sample tissues were processed by asepsis work,virus was inoculated in F81 cells and identified by electron microscopy,serology,molecular biology and challenge test.The results indicated that the virus produced typical CPE on F81 cells and the HA blood coagulation rate was 1:256.The diameter of virus particle was about 20 nm observed by electron microscopy.The specific band was at 570 bp in PCR identification which proved that the virus isolated from the samples was CPV,and named CPV-BJ03/17.The results of whole-genome sequencing and analysis indicated that the full length virus genome was 4 620 bp,GenBank accession No.:MF134808,and this virus strain had a closer relationship with virus isolated from Guangdong (SC02/2011),Shenzhen (CPV-s5) and Gansu (CPV-LZ1) whose nucleotide identities were all 99.7%.The analysis of VP2 amino acid sequence suggested that CPV-BJ03/17 belonged to a New CPV-2a subtype.The major amino acid sites of CPV-BJ03/17 showed no significant changes compared with the recent isolated stain BJ15-1.Animal experiments showed that CPV-BJ03/17 was a high CPV virulent strain.In conclusion,a high CPV virulent strain was isolated in this study,according to the comparison of CPV prevalence and genetic variation,this results also provided a reference for CPV disease treatment and vaccine research.

Key words: canine parvovirus (CPV); isolation and identification; sequence analysis

CLC Number:

S852.65⁺9.2

WANG Yang, HU Bo, LU Rongguang, LV Shuang, ZHAO Hui, LIAN Shizhen, YAN Xijun. Isolation and Identification of Canine Parvovirus CPV-BJ03/17 Strain and Genetic Evolution Analysis of VP2 Gene[J]. , 2018, 45(7): 1933-1941.

References

[1] PARKER J,MURPHY M,HUEFFER K,et al.Investigation of a canine parvovirus outbreak using next generation sequencing[J]. Scientific Reports,2017,7(1):9633.
[2] GUMBRELL R C.Parvovirus infection in dogs[J].New Zealand Veterinary Journal,1979,27(6):113.
[3] SAXENA L,KUMAR G R,SAXENA S,et al.Apoptosis induced by NS1 gene of canine parvovirus-2 is caspase dependent and p53 independent[J].Virus Research,2013,173(2):426-430.
[4] RAJ J M,MUKHOPADHYAY H K,THANISLASS J,et al.Isolation,molecular characterization and phylogenetic analysis of canine parvovirus[J].Infection,Genetics and Evolution,2010,10(8):1237-1241.
[5] MILLER C L,PINTEL D J.Interaction between parvovirus NS2 protein and nuclear export factor Crm1 is important for viral egress from the nucleus of murine cells[J].Journal of Virology,2002,76(7):3257-3266.
[6] XU J,GUO H C,WEI Y Q,et al.Phylogenetic analysis of canine parvovirus isolates from Sichuan and Gansu provinces of China in 2011[J].Transboundary and Emerging Diseases,2015,62(1):91-95.
[7] CSAGOLA A,VARGA S,LORINCZ M,et al.Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary[J].Archives of Virology,2014,159(9):2441-2444.
[8] VANNAMAHAXAY S,VONGKHAMCHANH S,INTANON M,et al.Molecular characterization of canine parvovirus in Vientiane,Laos[J]. Archives of Virology,2017,162(5):1355-1361.
[9] DECARO N,ELIA G,CAMPOLO M,et al.New approaches for the molecular characterization of canine parvovirus type 2 strains[J].Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health,2005,52(7-8):316-319.
[10] DECARO N,MARTELLA V,DESARIO C,et al.First detection of canine parvovirus type 2c in pups with haemorrhagic enteritis in Spain[J].Journal of Veterinary Medicine Series B-Infectious Diseasesand Veterinary Public Health,2006,53(10):468-472.
[11] NOOKALA M,MUKHOPADHYAY H K,SIVAPRAKASAM A,et al.Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India[J].Acta Microbiologica et Immunologica Hungarica,2016,63(4):411-426.
[12] ZHAO Y,LIN Y,ZENG X,et al.Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing,China[J]. Virology Journal,2013,10(1):272.
[13] FIGUEIREDO J,MIRANDA C,SOUTO R,et al.Genetic characterization of canine parvovirus type 2 subtypes in Maputo,Mozambique[J].Archives of Microbiology,2016,199(4):543-549.
[14] AITMAN K D,KELMAN M,WARD M P.Are vaccine strain,type or administration protocol risk factors for canine parvovirus vaccine failure[J].Veterinary Microbiology,2017,210:8-16.
[15] 张海玲,闫喜军,柴秀丽,等.水貂肠炎细小病毒PCR检测方法的建立和应用[J].特产研究,2007,29(2):1-3. ZHANG H L,YAN X J,CHAI X L,et al.Establishment and application of PCR for detection of mink enteritis virus[J].Special Wild Economic Animal and Plant Research,2007,29(2):1-3.(in Chinese)
[16] 仝明薇,易立,程世鹏.犬细小病毒JL-M13株的分离鉴定及全基因组序列分析[J].动物医学进展,2015,36(1):19-22. TONG M W,LI Y I,CHENG S P.Isolation,identification and sequence analysis of complete genome of canine parvovirus JL-M13 strain[J].Progress in Veterinary Medicine,2015,36(1):19-22.(in Chinese)
[17] MIRANDA C,THOMPSON G.Canine parvovirus:The worldwide occurrence of antigenic variants[J].Journal of General Virology,2016,97(9):2043-2057.
[18] WOOLFORD L,CROCKER P,BOBROWSKI H,et al.Detection of the canine parvovirus 2c subtype in Australian dogs[J].Viral Immunology,2017,30(5):371-376.
[19] TRUYEN U.Evolution of canine parvovirus-A need for new vaccines[J].Veterinary Microbiology,2006,117(1):9-13.
[20] SMITH K,WU M A O,LUO M,et al.The three-dimensional structure of canine parvovirus and its functional implications[J].Science,1991,251(5000):1456-1464.
[21] 张传鹏,张航,高全新,等.犬细小病毒SH15株的分离鉴定与全基因组分析[J].中国兽医科学,2017,47(2):165-171. ZHANG C P,ZHANG H,GAO Q X,et al.Isolation and identification of canine parvovirus SH15 strain and sequence analysis of its complete genome[J].Chinese Veterinary Science,2017,47(2):165-171.(in Chinese)
[22] HAN S C,GUO H C,SUN S Q,et al.Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China[J].Archives of Microbiology,2015,197(4):621-626.
[23] 刘金凤,廖文军,陈凤莲,等.犬细小病毒广西流行株的分离鉴定及生物学特性分析[J].中国兽医科学,2017,47(7):877-883. LIU J F,LIAO W J,CHEN F L,et al.Isolation and identification of canine parvovirus isolated from Guangxi and analysis of it's biological characteristics[J].Chinese Veterinary Science, 2017,47(7):877-883.(in Chinese)
[24] MIRA F,PURPARI G,LORUSSO E,et al.Introduction of Asian canine parvovirus in Europe through dog importation[J].Transboundary and Emerging Diseases,2018,65(1):16-21.

Isolation and Identification of Canine Parvovirus CPV-BJ03/17 Strain and Genetic Evolution Analysis of VP2 Gene

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