China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (12): 3861-3869.doi: 10.16431/j.cnki.1671-7236.2020.12.008

• Biotechnology • Previous Articles     Next Articles

Bioinformatics Analysis of Feline importin Gene and Its Dynamic Expression in F81 Cells Infected with Canine Parvovirus

ZHAO Hang1, SONG Shanshan2, WANG Jianke3, LIN Peng4   

  1. 1. Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Laboratory of Agro-food Safety and Quality, Institute of Food Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Jilin Province State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    3. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    4. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, National R & D Branch Center of Surimi and Surimi Products Processing, College of Food Science and Technology, Bohai University, Jinzhou 121013, China
  • Received:2020-07-06 Online:2020-12-20 Published:2020-12-18

Abstract: This study was aimed to systematically analyze the gene sequences and proteins of the importin subtype genes (importin α1,importin α3,importin α4,importin α5,importin α6,importin α7,importin α8 and importin β),and the change of transcription level of importin subtype of F81 cells infected CPV.The full length of importin genes were amplified by RT-PCR techniques from F81 cells and the amino acid sequence and the structure and function of importin protein were subsequently analyzed by bioinformatics software.Meanwhile,the dynamic change of expression of importin genes in CPV-infected F81 cells were investigated at 24,48 and 72 h postinfection by Real-time RT-PCR.The results showed that full length of the importin α subtype genes were about 1 500 bp and the importin β was 2 600 bp.The physical chemical properties of importin proteins showed the theoretical isoelectric was about 4.60 and the importin α1,importin α5,importin α6,importin α7 and importin β belonged to instable proteins.Meanwhile,there were no signal peptide,no transmembrane structures and no cellular localization in importin proteins.The secondary structure of importin proteins were predicted by Predictprotein software and mainly composed of α-helix and random coil.Real-time RT-PCR results showed that the importin α1 (P<0.01) and importin β mRNA level were significantly reduced in CPV-infected F81 cells,while other importin mRNA level were markedly up-increased,the importin α8 gene had the highest expressional level (P<0.01).The study laid a foundation for further study on the transport mechanism of CPV into the nucleus of the cell and developing drugs.

Key words: importin; canine parvovirus (CPV); sequence analysis; expression difference

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