松辽黑猪FOXA2基因第3外显子多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.016

Abstract

Abstract: 【Objective】 The aim of this study was to analyze the genetic diversity of forkhead box A2 (FOXA2) gene exon 3 polymorphism in local pig breeds and its correlation with reproductive traits in Songliao Black pigs.【Method】 The single nucleotide polymorphisms (SNPs) (genotype frequency,gene frequency,genetic homozygosity,genetic heterozygosity,number of effective alleles,polymorphic information content) of FOXA2 gene exon 3 were screened by direct sequencing in ear tissues of 90 healthy Songliao Black pigs,and the correlation between SNP of FOXA2 gene eoxn 3 and reproductive performances (total number of litter,number born alive,number of nipples,birth weight,3-week weight,weaning weight and weaned piglets) were analyzed using SPSS 26.0 software.【Result】 The results obtained that SNPs sites (C1337A,C1343G,C1626T)were found of FOXA2 gene exon 3 in Songliao Black pig.Genotypes of CC,CA and AA were contained in C1337A,genotypes of CC,CG and GG were contained in C1343G,and genotypes of CC,CT and TT were contained in C1626T.The chi-square fitness test showed that SNPs (C1337A,C1626T) were in Hardy-Weinberg equilibrium (P＞0.05),C1343G was not in Hardy-Weinberg equilibrium (P<0.05).The calculation of polymorphism information content (PIC) showed that the genetic heterozygosities of C1337A and C1626T were in moderate polymorphism (0.25＜PIC＜0.50),the genetic heterozygosity of C1343G was in the highly polymorphism (PIC＞0.50).The results of association analysis showed that the total number born of CC genotype with C1337A locus was significantly higher than that of CA genotype (P＜0.05),the 3-week weight and weaning weight of piglets born by AA genotype individuals were extremely significantly higher than that of CC and CA genotype (P＜0.01);The 3-week weight and weaning weight of piglets born by CC genotype individuals with C1626T locus were significantly or extremely significantly higher than that of CT and TT genotype (P＜0.05 or P＜0.01),the number of nipples of CC genotype was significantly higher than that of CT genotype (P＜0.05).【Conclusion】 There were three SNPs sites (C1337A,C1343G,C1626T) in exon 3 of FOXA2 gene,which had a significant correlation with the total number born,3-week weight,weaning weight and number of nipples of Songliao Black pig.

Key words: Songliao Black pig; FOXA2 gene; exon 3; reproductive performances; correlation analysis; genetic markers

CLC Number:

S813.3

ZHANG Fangwei, ZHANG Qi, ZHANG Yunpeng, LI Xin, ZHANG Jingbo, ZHANG Shumin, YU Yongsheng. Polymorphism of FOXA2 Gene Exon 3 and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1444-1451.

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Polymorphism of FOXA2 Gene Exon 3 and Its Association with Reproductive Traits in Songliao Black Pigs

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