松辽黑猪METTL23基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.12.021

Abstract

Abstract: In order to investigate the polymorphism of METTL23 gene and its association with reproductive traits in Songliao Black pig.178 Songliao Black sows were selected as the research objects.The single nucleotide polymorphisms (SNPs) of exons 1-5 of METTL23 gene were screened by Sanger direct sequencing, and the correlation between SNP of METTL23 gene and reproductive traits (total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglet number and nipple number) were analyzed by using SPSS 19.0 software.The results showed that only one SNP site (named A62G) was found in exon 4 of METTL23 gene in Songliao Black pig.There were three genotypes (AA, AG and GG) in the research population.GG and G were dominant genotype and allele, which was in Hardy-Weinberg balanced (P>0.05).The genetic homozygosity (Ho) of this locus was 0.6516 which was higher than that of genetic heterozygosity (He) of 0.3484, indicating that it had little variation in the population of Songliao Black pigs.The polymorphism information content (PIC) was 0.2877, which belonged to moderate polymorphism (0.25<PIC<0.5), indicating that the genetic marker could provide a certain amount of genetic information.Correlation analysis results showed that the total litter size, live litter size and weaned piglets of GG genotype were significantly or extremely significantly higher than those of AA genotype (P<0.05; P<0.01), and the total litter size was also significantly higher than that of AG genotype individuals (P<0.05).The number of weaned piglets in AG genotype was significantly higher than that in AA genotype (P<0.05).There were no significant differences among genotypes in birth weight, 3-week-old weight, weaning weight and nipple number (P>0.05).In conclusion, there was a SNP site A62G in exon 4 of METTL23 gene, which was significantly correlated with the litter size and might be a genetic marker for litter size in Songliao Black pig molecular breeding.

Key words: Songliao Black pig; METTL23 gene; reproductive traits; correlation analysis

CLC Number:

S813.3

ZHANG Qi, LI Na, YU Yongsheng, LIU Qingyu, ZHANG Qing, GAO Yi, LIU Hongliang, ZHANG Yunpeng, ZHANG Jingbo, ZHANG Shumin. Polymorphism of METTL23 Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4530-4536.

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Polymorphism of METTL23 Gene and Its Association with Reproductive Traits in Songliao Black Pigs

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