松辽黑猪RBP4基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.08.017

Abstract

Abstract: This study was aimed to investigate the association of the polymorphism of retinol-binding protein 4 (RBP4) gene with reproductive traits in Songliao Black pigs.132 Songliao Black pigs were selected,the single nucleotide polymorphism (SNP) of RBP4 gene in Songliao Black pigs were screened by PCR-HRM combined with DNA sequencing,and the correlation between SNPs and reproductive traits (total number born,number born alive,number of nipples,birth weight,3-week weight and weaning weight) were analyzed.The results showed that two SNPs (G45A and C170T) of RBP4 gene were detected,which were located in exons 3 and 5,respectively.There were three genotypes (GG,GA and AA) in the G45A site,and two genotypes (CC and CT) in the C170T site.The G45A site was uniform in Songliao Black pigs,and its variation and polymorphism were moderate,while it was not in Hardy-Weinberg equilibrium (P<0.01).The C170T site was uneven in Songliao Black pigs,and its variation and polymorphism were low,it was in Hardy-Weinberg equilibrium (P>0.05).In the G45A site,the total number born and number born alive were showed as GG > GA > AA,the total born and number born alive of GG genotype were significantly higher than AA genotype (P<0.05),the 3-week weight of GG genotype was significantly lower than GA genotype (P<0.05),the weaning weight of GG genotype was significant lower than GA and AA genotypes (P<0.05),there was no significant difference of birth weight and number of nipples in different genotypes (P>0.05).In the C170T site,there was no significant difference of all reproductive traits in different genotypes (P>0.05).In conclusion,the G45A site of RBP4 gene had a great influence on the litter size in Songliao Black pigs,it needed to further explore whether the G45A site could be used as a genetic marker for the litter size in Songliao Black pigs.The C170T site might not be a mutation site associated with reproductive traits in Songliao Black pigs,it needed to expand the number of pig for further verify.

Key words: Songliao Black pigs; RBP4 gene; reproductive traits; association analysis

CLC Number:

S813.3

WANG Wanxing, ZHANG Qi, ZHANG Shumin. Polymorphism of RBP4 Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2493-2501.

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Polymorphism of RBP4 Gene and Its Association with Reproductive Traits in Songliao Black Pigs

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