中国不同地区羊吕氏泰勒虫分子流行病学调查及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2020.06.030

Abstract

Abstract: To understand the prevalence of T.luwenshuni in goats and sheep in different regions of China,in this study,281 sheep blood samples collected from 7 regions of Henan,Gansu,Shaanxi,Shanxi,Guizhou,Yunnan and Xinjiang were tested by PCR based on the 18S rRNA gene locus of T.luwenshuni,and positive samples were sequenced for sequence analysis.The results showed that the total infection rate of T.luwenshuni was 35.23% (99/281).The infection rate of T.luwenshuni differed significantly among sampling sites (P<0.01),among them,the infection rate of T.luwenshuni in Henan province was the highest (98%,49/50),and the lowest in Xinjiang (0).The infection rates of T.luwenshuni in sheep and goats were 51.67% (31/60) and 30.77% (68/221),respectively,and the differences were extremely significant (P<0.01).The infection rate of T.luwenshuni in grazing sheep (41.55%,86/207) was significantly higher than that in stabling sheep (17.57%,13/74) (P<0.01).The infection rates of T.luwenshuni were 56.00% (28/50),42.75% (59/138),9.43% (5/53) and 17.50% (7/40) in spring,summer,autumn and winter,respectively,and the differences were extremely significant (P<0.01).The infection rates of T.luwenshuni in sheep aged ≥ 12 months and <12 months were 33.50% (67/200) and 39.51% (32/81),respectively,with no significant difference (P>0.05).In addition,the genetic and evolutionary analysis indicated that the isolates of T.luwenshuni obtained from this study were more than 99.60% homologous to the isolates of T.luwenshuni isolated from China and they were located on the same cluster.This survey provided an important reference for further understanding of the prevalence and distribution of T.luwenshuni in sheep and goats in different regions of China.

Key words: T.luwenshuni; epidemiology; 18S rRNA gene; genetic and evolutionary analysis; sheep; goats

CLC Number:

S852.7⁺1

CHEN Qian, WANG Kunlun, YAN Yaqun, ZHOU Yongchun, ZHAO Cong, JIAN Fuchun, WANG Rongjun, ZHANG Longxian, NING Changshen. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Theileria luwenshuni in Sheep and Goats in Different Regions of China[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1902-1909.

References

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Molecular Epidemiological Investigation and Genetic Evolution Analysis of Theileria luwenshuni in Sheep and Goats in Different Regions of China

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