安卡拉病毒贵州流行株penton基因分子特性分析

doi:10.16431/j.cnki.1671-7236.2020.06.029

Abstract

Abstract: To understand the molecular characteristics of penton gene of Ankara virus Guizhou strains,specific primer was designed and synthesized based on the sequence of FAdV gene,and the penton gene CDS regions were amplified.The nucleotide sequence,physical and chemical properties of protein,signal peptide,secondary structure,tertiary structure and B cell epitope of penton gene were analyzed by bioinformatics software.The results showed that three FAdV (GZ-BJ,GZ-QL and GZ-LPS) penton gene fragments were 1 578 bp.Phylogenetic tree analysis showed that GZ-BJ,GZ-QL and GZ-LPS all belonged to FAdV-4 type.The nucleotide homology of three FAdV-4 strains ranged from 99.9% to 100%,and the amino acid sequence homology ranged from 99.8% to 100%.There was a little difference with other domestic isolates and foreign isolates.The physical and chemical properties of the protein showed that the protein was unstable and had no transmembrane structure.The secondary structure was mainly composed of random coil (54.29%).The domain prediction results showed that the penton protein contained only a low complexity region.B cell epitope prediction showed that there were three dominant epitopes in the amino acid encoded by penton gene:5-171,207-236 and 420-462.The results indicated that the Ankara virus penton gene was relatively conserved and there were multiple dominant epitopes.It could be used as a target protein for Ankara virus prevention and control research,laying a foundation for the virus prevention and control and pathogenic mechanism research.

Key words: Ankara virus; penton gene; clone; bioinformatic analysis

CLC Number:

S852.65⁺9.1

HE Ling, ZHANG Yundan, LI Qiaobin, YANG Yuan, ZHOU Bijun, WEN Ming, CHENG Zhentao, YUE Jun. Molecular Characteristics Analysis of penton Gene of Ankara Virus Guizhou Strains[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1892-1901.

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Molecular Characteristics Analysis of penton Gene of Ankara Virus Guizhou Strains

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