China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2633-2641.doi: 10.16431/j.cnki.1671-7236.2019.09.017

• Genetics and Breeding • Previous Articles     Next Articles

Study on Genetic Diversity and System Evolution of mtDNA D-Loop Region in Xianglushan Chicken

LI Xingcai1,2, PAN Chengyong3, LI Hui1,2, YANG Huating1,2, CHEN Lin1,2, XIAO Tao1,2   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Key Laboratory of Animal Genetics, Breeding and Reproduction in Guizhou, Guizhou University, Guiyang 550025, China;
    3. Kaili Fenghuangyuan Animal Husbandry Breeding Limited in Guizhou, Kaili 556000, China
  • Received:2019-01-04 Online:2019-09-20 Published:2019-09-21

Abstract: This study was aimed to analyze the genetic diversity and phylogenetic evolution in Xianglushan chicken.The full-length sequences of mitochondrial DNA D-Loop (mtDNA D-Loop) region of 121 Xianglushan chickens were analyzed by PCR amplification combined with bidirectional sequencing,and the composition,variation and maternal origin of mtDNA D-Loop region were discussed.The results showed that the total length of mtDNA D-Loop region in Xianglushan chicken was 1 231-1 233 bp.The contents of A,T,C and G were 26.62%,33.55%,26.49% and 13.34%,respectively.The content of T was the highest,the content of G was the lowest,and the content of A+T was significantly higher than that of G+C,it indicated that region might have certain base hobbies.Analysis of 121 full-length sequences were found to coexist in 11 haplotypes and 26 mutation sites,of which 2 were single polymorphic information sites,24 were simple information sites,4 bases were inserted and 2 bases were missing.The genetic diversity analysis results showed that the haplotype diversity was 0.814,the nucleotide diversity was 0.00447,the average nucleic acid difference was 5.494,which indicated that the genetic diversity of Xianglushan chicken was relatively rich,the effect of preservation was better,which had a certain breeding space.Tajima's D was 0.39378 and the test results were not significant (P>0.10),in line with neutral mutations.The cluster analysis results showed that Xianglushan chicken and Gallus gallus gathered as one,indicating that Xianglushan chicken originated from Gallus gallus, and there were 4 branches inside the branch,indicating that Xianglushan chicken had many matrilineal origins.The results could provide some reference data for the protection and exploitation of pheasant germplasm resources in Xianglushan chicken.

Key words: Xianglushan chicken; mitochondrial DNA D-Loop (mtDNA D-Loop); genetic diversity; haplotype diversity

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