China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2625-2632.doi: 10.16431/j.cnki.1671-7236.2019.09.016

• Genetics and Breeding • Previous Articles     Next Articles

Polymorphism of ARID5B Gene and Its Correlation with Body Size and Meat Quality Traits in Yanhuang Cattle

HU Zhongchang1,2, CAO Yang1,2, WU Jian1,2, QIN Lihong1,2, JIN Haiguo1, ZHAO Yumin1,2   

  1. 1. Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling 136100, China;
    2. Key Laboratory of Beef Cattle Genetics and Breeding in Ministry of Agriculture and Rural Agriculture, Changchun 130033, China
  • Received:2019-01-23 Online:2019-09-20 Published:2019-09-21

Abstract: This study was aimed to investigate the polymorphism of AT-rich interactive domain 5B (ARID5B) gene and its correlation with the body size and meat quality traits in Yanhuang cattle.99 Yanhuang cattle at 18 months old were selected as the research object,the SNP of 13 exons of ARID5B gene was found using PCR sequencing,the polymorphism of SNP was analyzed by HRM typing,and the association between ARID5B gene polymorphism and the body size and meat quality traits in Yanhuang cattle was analyzed by SPSS 19.0 software.The results showed that A/G mutation was detected at Chr28:18186635 bp of exon 10 of ARID5B gene in Yanhuang cattle,which contained three genotypes:AA,AG and GG,AA genotype was the dominant genotype,and A was the dominant allele.The chi-square suitability test result showed that Yanhuang cattle population was in Hardy-Weinberg equilibrium (P>0.05),population genetic parameter analysis result showed that the heterozygosity of the mutation site was relatively low,and had a low varitation in Yanhuang cattle population;Polymorphism information content (PIC) analysis showed that it was a moderately polymorphic (0.25 < PIC < 0.5).The correlation analysis results showed that there was a significant difference between the GG genotype of exon 10 of ARID5B gene and the body height,chest circumference,abdominal circumference and intramuscular fat content in Yanhuang cattle (P<0.05).This experiment revealed the correlation between ARID5B gene and meat traits in cattle,which provided a reference for exploring the feasibility as an effective genetic marker,and provided experimental basis for molecular breeding of Yanhuang cattle.

Key words: Yanhuang cattle; ARID5B gene; polymorphisms; meat traits; genetic marker

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