东串猪Mx1基因第14外显子多态性及组织表达谱分析

doi:10.16431/j.cnki.1671-7236.2018.02.020

Abstract

Abstract:

In this study, the PCR-RFLP and Real-time quantitative PCR assays were performed to explore the tissue expression patterns of myxo-virus resistance 1 (Mx1) gene and identify the poly-morphisms of Mx1 gene exon 14 in Dongchuan pig. The results demonstrated that there was a G36T mutation at the Hin6 Ⅰ restriction site of Mx1 gene exon 14. Furthermore, three genotypes were detected and named as AA, AB and BB, AB genotype was the dominant genotype and A was the dominant gene. Genetic polymorphism analysis showed that the polymorphism information content (PIC) value of Mx1 gene in Dongchuan pig was 0.360, which displayed moderate polymorphism. The Mx1 gene had higher expression levels in lymphatic, lung, spleen, duodenum, jejunum and ileum.This study preliminarily revealed the polymorphism distribution of Mx1 gene exon 14 in Dongchuan pig, and provided a reference for its feasibility as an effective genetic marker. In addition, the high expression of Mx1 gene in the immune tissues might be associated with the resistance to pathogenic infections.

Key words: Dongchuan pigs; Mx1 gene; polymorphisms; expression profile

CLC Number:

S813.3

WU Jiayun, ZHU Enhao, WANG Haifei, WU Shenglong, BAO Wenbin. Polymorphism and Tissue Expression Profile of Mx1 Gene Exon 14 in Dongchuan Pigs[J]. , 2018, 45(2): 448-455.

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Polymorphism and Tissue Expression Profile of Mx1 Gene Exon 14 in Dongchuan Pigs

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