槟榔江水牛OXCT1基因的分子特征及组织表达谱分析

doi:10.16431/j.cnki.1671-7236.2019.06.002

Abstract

Abstract:

In this study,the complete CDS of 3-oxoacid CoA-transferase 1 (OXCT1) gene in Binglangjiang buffalo was amplified by PCR method,and the gene functional bioinformatics and tissue differential expression were analyzed.Specific primers were designed by Primer Premier 5.0 according the sequence of Bos taurus OXCT1 gene in GenBank (accession No.:XM_006076397),genomic DNA was extracted from Binglangjiang buffalo as template,the mRNA sequence of buffalo OXCT1 gene was amplified by PCR,the amplification products were sequenced and identified by open reading frame (ORF) with ORF Finder software,and then obtained complete sequence of OXCT1 gene CDS in buffalo which was analyzed by bioinformatics.The results showed that the length of OXCT1 gene CDS was 1 563 bp which encoded 520 amino acids,and the protein molecular formula was C₂₅₀₉H₄₀₄₁N₆₈₇O₇₄₆S₂₃,the molecular weight was 56.50 ku,the theory isoelectric point (pI) was 8.69,the instability coefficient was 27.49,the grand average of hydropathicity was -0.097,and the OXCT1 was the weak hydrophilic protein.The OXCT1 protein had no signal peptide and transmembrane structure,so it belonged to mitochondrial membrane protein.Buffalo OXCT1 contained two conserved domains which named as pcaJ_scoB_fam and AtoD.The homology of OXCT1 amino acid sequences was ≥ 97% between buffalo and other species such as cattle,Tibetan antelope,etc.The expression profile of buffalo OXCT1 gene in 13 tissues was assayed in the stages of lactation and non-lactation.The results showed that the expression level of OXCT1 gene was the highest in mammary gland,but it was not expressed in kidney,pituitary,fat,brain,skin and muscle in lactation.In non-lactating stage,the OXCT1 gene was found to express in all tissues except fat.The OXCT1 gene had the highest expression level of mammary gland in buffalo lactating,which suggested that OXCT1 gene might be associated with the regulation of milk fat synthesis.The results would provide a basis to further understanding about the anabolism and regulation mechanism of milk fat.

Key words: Binglangjiang buffalo; OXCT1 gene; cloning; bioinformatics; tissue expression profile

CLC Number:

Q786

HE Qing, LIU Qin, HA Fu, ZHANG Yongyun, MIAO Yongwang. Molecular Characterization and Tissue Expression Profile of OXCT1 Gene in Binglangjiang Buffalo[J]. , 2019, 46(6): 1576-1584.

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Molecular Characterization and Tissue Expression Profile of OXCT1 Gene in Binglangjiang Buffalo

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