CREB-H基因在猪不同组织中表达谱及肝脏中的表达规律

doi:10.16431/j.cnki.1671-7236.2019.09.006

Abstract

Abstract: The aim of this study was to investigate the expression characteristics of cyclic AMP-responsive elmment-binding protein 3-like 3 (CREB-H) in different tissues of pig and the expression patterns of CREB-H in liver of Mashen and Large White pigs.Real-time quantitative PCR and Western blotting were used to detect the expression profile of CREB-H gene in 12 tissues (heart,liver,spleen,lung,kidney,stomach,small intestine,cerebellum,hypothalamus,longissimus dorsi muscle,biceps femoris and psoas major) of Mashen pig and the expression patterns of CREB-H in liver of Mashen and Large White pigs at 1,30,60,90,120,150 and 180 days of age.The results showed that CREB-H gene mRNA was widely expressed in 12 tissues of sampled pigs,and the expression was high in liver and small intestine.The expression of CREB-H protein in liver was significantly higher than that in other tissues (P<0.05),and it was not expressed in heart,spleen and cerebellum.The developmental expression patterns of CREB-H gene mRNA and protein occurring in liver of pigs were affected by days of age,breed and the interaction of breed and days of age (P<0.01).The expression of CREB-H gene mRNA and protein in liver were the highest at 1 day of age after birth between two breeds,and the expression of CREB-H protein in Mashen pig was extremely significantly higher than that in Large White pig at each stage (P<0.01),and CREB-H was mainly expressed in liver of pig.There were temporal and spatial differences of the expression in liver between Mashen and Large White pigs,which might be relate to lipid metabolism in pigs at different developmental stages,this results provided a theoretical basis for studying the regulation mechanism of lipid metabolism in pigs.

Key words: pig; CREB-H gene; liver; expression profile; developmental expression patterns

CLC Number:

Q786

GUO Xiaohong, LIU Cuicui, LI Meng, ZHANG Wanfeng, QIN Benyuan, LIU Min, CAI Chunbo, GAO Pengfei, CAO Guoqing, LI Bugao. Expression Profile and Patterns of CREB-H Gene in Different Tissues and Liver of Pig[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2543-2550.

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Expression Profile and Patterns of CREB-H Gene in Different Tissues and Liver of Pig

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