猪PAANCR组织表达谱分析及其对脂质生成的影响

doi:10.16431/j.cnki.1671-7236.2019.10.003

Abstract

Abstract: In previous work,a novel lncRNA (PAANCR) was found to significantly associated with porcine lipogenesis,so the aim of this study was to investigate the expression of PAANCR in porcine different tissues and fatten periods and its relationship with lipogenesis.10 healthy Landrace×Large White piglets were selected,3 pigs were randomly selected and slaughtered at 90 and 210 days,respectively.Real-time PCR was used to detected the expression files of PAANCR,meanwhile Oil Red O staining and Real-time PCR were used to analyze lncRNA_{B130024G19Rik}'s (a mouse lncRNA,the homologue of PAANCR) effect on lipid deposition in 3T3-L1 preadipocyte.The results showed that the expression trends of PAANCR was tissue- and development-specific,its expressional level was higher in lung,uterus,spleen,kidney and adipose tissues,its expression levels was up-regulated in liver,small intestine and brain during the development,which couldn't be detected in muscle.During differentiation,when the expression of lncRNA_{B130024G19Rik} was knockdown,the expression level of genes associated with adipogenesis and lipid deposition were down-regulated.The results indicated that PAANCR could be used as candidate factor for porcine lipogenesis,and it was meaningful for understanding the molecular mechanism of lncRNA on lipogenesis.

Key words: pig; lncRNA; tissue expression profile; RNA interference; lipogenesis

CLC Number:

WANG Jing, LI Qingdong, HUA Liushuai, CHEN Junfeng, ZHANG Jiaqing, REN Qiaoling, LIU Fujiu, ZHANG Hua, CAO Hai, XU Zhaoxue, XING Baosong, BAI Xianxiao. Expression Profile Analysis of Porcine PAANCR and Its Effect on Lipogenesis[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2834-2842.

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Expression Profile Analysis of Porcine PAANCR and Its Effect on Lipogenesis

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