水牛PPARG来源环状RNA的鉴定及表达谱分析

doi:10.16431/j.cnki.1671-7236.2019.12.001

Abstract

Abstract: To evaluate the results of transcriptome sequencing analysis and explore circular RNA(circRNA) with potential function in fat deposition,10 circRNA derived from the peroxisome proliferator activated receptor γ (PPARG) were identified.Based on the obtained sequence,divergent primers were designed for RT-PCR and normal sequencing to obtain real circRNA.Real-time PCR was used to analyze the expression profile of circRNA in different tissues and adipose tissue of different parts in buffalo,RT-PCR was used to identify their expression in different species.In total,5 of the 10 PPARG-derived circRNA were identified,and their sequences were consist with those in transcriptome sequencing analysis.Specific primers for Real-time PCR were available for only 2 of the 5 circRNA,and the 2 circRNA were mainly expressed in adipose tissue and with different expression levels among adipose tissue depots.Further,3 circRNA were detectable in adipose tissues of cattle,yak,pig and mouse.Their length were consist with those in buffalo,and they were with high similarity in sequence among species.These results indicated that the results of transcriptome sequencing analysis were reliable,2 circRNA were mainly expressed in adipose tissue and conserved among species,which could be candidate circRNA for fat deposition of buffalo.

Key words: buffalo; PPARG gene; circular RNA; identification; expression profile

CLC Number:

Q786

FENG Xue, HONG Huiying, SUN Xiaofei, QU Kaixing, HUANG Bizhi, WEI Xuefeng, MA Yun, HUANG Jieping. Identification and Expression Profile Analysis of PPARG-derived Circular RNA in Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3457-3465.

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Identification and Expression Profile Analysis of PPARG-derived Circular RNA in Buffalo

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