延边黄牛ATF3基因组织表达及其多态性与肉质性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.02.020

Abstract

Abstract: The aim of this study was to elucidate the association between tissue expression level and polymorphism of ATF3 gene and meat quality traits in Yanbian Yellow cattle.Using 70 healthy 30-month-old Yanbian Yellow cattle as the research object,Real-time PCR was used to compare the expression of ATF3 gene in 10 tissues including heart,liver,spleen,lung,kidney,hind leg muscle,eye muscle,sirloin,upper brain and subcutaneous fat tissue.The exon 4 of ATF3 gene was amplified,SNP was screened and verified by Sanger direct sequencing and PCR-RFLP methods,and the association with meat quality traits was analyzed.The results showed that the expression of ATF3 gene was detected in 10 tissues of Yanbian Yellow cattle,and the expression of ATF3 gene in subcutaneous adipose was extremely significantly higher than other tissues (P < 0.01).The relative expression of ATF3 gene in sirloin was extremely significantly positively correlated with IMF content (P < 0.01),and the coefficient of correlation was 0.996;There was a significant positive correlation between ATF3 gene expression and IMF content in eye muscle (P < 0.05),and the coefficient of correlation was 0.999.There were no significant correlation between the expression of ATF3 gene and IMF content in the upper brain and hind leg muscle (P > 0.05),and the correlation coefficients were 0.757 and 0.658,respectively.There was a G→C mutation at 1 428 bp of ATF3 gene,contained three genotypes:GG,GC and CC.The fat content and back fat thickness of GC and CC genotypes were significantly higher than GG genotype (P < 0.05),the marbling grade of GC and CC genotypes were extremely significantly better than GG genotype (P<0.01).The results demonstrated that the mutation site could be used as a potential molecular marker for meat quality traits,and ATF3 gene might be a candidate gene for fat deposition in Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; ATF3 gene; tissue expression profile; polymorphism; meat quality traits

CLC Number:

S813.3

SHAO Jing, YIN Baozhen, JIAO Shi, ZHANG Jiasu, ZHANG Luomeng, GENG Chunyin, XIA Guangjun. A Tissue Expression and Polymorphism of ATF3 Gene and Its Association with Meat Quality Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 488-496.

References

[1] HAI T,WOLFGANG C D,MARSEE D K,et al.ATF3 and stress responses[J].Gene Expression,1999,7(4-6):321-335.
[2] HAI T,HARTMAN M G.The molecular biology and nomenclature of the activating transcription factor/cAMP responsive element binding family of transcription factors:activating transcription factor proteins and homeostasis[J].Gene (Amsterdam),2001,273(1):1-11.
[3] THOMPSON M R,XU D,WILLIAMS B R G.ATF3 transcription factor and its emerging roles in immunity and cancer[J].Journal of Molecular Medicine,2009,87(11):1053-1060.
[4] 崔畅,范志祥,钱忠义,等.ATF3基因在正常小鼠脑组织中的表达定位研究[J].云南大学学报(自然科学版),2018,40(4):814-819. CUI C,FAN Z X,QIAN Z Y,et al.Localization and expression of ATF3 gene in unstimulated mouse brain[J].Journal of Yunnan University (Natural Sciences Edition),2018,40(4):814-819.(in Chinese)
[5] YIN X,DEWILLE J W,HAI T.A potential dichotomous role of ATF3,an adaptive-response gene,in cancer development[J].Oncogene,2008,27(15):2118-2127.
[6] GOKULNATH M,SWETHA R,THEJASWINI G,et al.Transforming growth factor-β1 regulation of ATF-3,c-Jun and JunB proteins for activation of matrix metalloproteinase-13 gene in human breast cancer cells[J].International Journal of Biological Macromolecules,2017,94:370-377.
[7] LAN Y F,CHEN H H,LAI P F,et al.microRNA-494 reduces ATF3 expression and promotes AKI[J].Journal of the American Society of Nephrology,2012,23(12):2012-2023.
[8] HAYNER J N,SHAN J,KILBERG M S.Regulation of the ATF3 gene by a single promoter in response to amino acid availability and endoplasmic reticulum stress in human primary hepatocytes and hepatoma cells[J].Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms,2018,1861(2):72-79.
[9] GREEN T A,ALIBHAI I N,UNTERBERG S,et al.Induction of activating transcription factors (ATFs) ATF2,ATF3,and ATF4 in the nucleus accumbens and their regulation of emotional behavior[J].Journal of Neuroscience,2008,28(9):2025-2032.
[10] LEE Y S,KOBAYASHI M,KIKUCHI O,et al.ATF3 expression is induced by low glucose in pancreatic α and β cells and regulates glucagon but not insulin gene transcription[J].Endocrine Journal,2014,61(1):85-90.
[11] KIYOSHI T,BAYIN H,SUSUMU A,et al.Stress response gene ATF3 is a target of c-myc in serum-induced cell proliferation[J].EMBO Journal,2014,24(14):2590-2601.
[12] GU L,GE Z,WANG Y,et al.Activating transcription factor 3 promotes intestinal epithelial cell apoptosis in Crohn's disease[J].Pathology-Research and Practice,2018,214(6):862-870.
[13] CHAN J,JCC T,PTW L,et al.RNA-seq revealed ATF3-regulated inflammation induced by silica[J].Toxicology,2018,393:34-41.
[14] KIM J Y,PARK K J,HWANG J Y,et al.Activating transcription factor 3 is a target molecule linking hepatic steatosis to impaired glucose homeostasis[J].Journal of Hepatology,2017,67(2):349-359.
[15] INOUE M,UCHIDA Y,EDAGAWA M,et al.The stress response gene ATF3 is a direct target of the Wnt/β-catenin pathway and inhibits the invasion and migration of HCT116 human colorectal cancer cells[J].PLoS One,2018,13(7):e0194160.
[16] KIM H B,KONG M,KIM T M,et al.NFATc4 and ATF3 negatively regulate adiponectin gene expression in 3T3-L1 adipocytes[J].Diabetes,2006,55(5):1342-1352.
[17] JANG M K,KIM C H,SEONG J K,et al.ATF3 inhibits adipocyte differentiation of 3T3-L1 cells[J].Biochemical & Biophysical Research Communications,2012,421(1):38-43.
[18] JANG M K,JUNG M H.ATF3 represses PPARγ expression and inhibits adipocyte differentiation[J].Biochemical and Biophysical Research Communications,2014,454(1):58-64.
[19] KIM S,SONG N J,BAHN G,et al.ATF3 induction is a therapeutic target for obesity and metabolic diseases[J].Biochemical and Biophysical Research Communications,2018,504(4):903-908.
[20] RUIZ-JIMÉNEZ J,PRIEGO-CAPOTE F,CASTRO M D L D.FT-midIR determination of fatty acid profiles,including trans fatty acids,in bakery products after focused microwave-assisted Soxhlet extraction[J].Analytical and Bioanalytical Chemistry,2006,385(8):1532-1537.
[21] 罗小芬.浅谈凯氏定氮法测定蛋白质准确度的操作技巧及关键点[J].现代食品,2017,10(19):99-100. LUO X F.Talking about the operation skills and key points of determining protein accuracy by Kjeldahl method[J].Modern Food,2017,10(19):99-100.(in Chinese)
[22] 郭盼盼,毛元廷,李香子,等.延边黄牛公牛与阉牛牛肉营养成分比较研究[J].中国畜牧兽医,2016,43(9):2333-2338. GUO P P,MAO Y T,L X Z,et al.Comparison research on nutrient composition of meet between Yanbian Yellow bulls and steers[J].China Animal Husbandry & Veterinary Medicine,2016,43(9):2333-2338.(in Chinese)
[23] 董玉影,孙晓龙,金颖,等.延边黄牛产业发展的思考与展望[J].肉类研究,2014,6:30-33. DONG Y Y,SUN X L,JIN Y,et al.Yellow cattle industry in Yanbian,Northeast China's Jilin province:Development and prospects[J].Meat Research,2014,6:30-33.(in Chinese)
[24] 庞喆.基于RNA-seq延边黄牛[候选基因的筛选与表达分析[D].延吉:延边大学,2018. PANG Z.Screening and expression analysis of intramuscular fat candidate genes in Yanbian Yellow cattle based on RNA-seq[D].Yanji:Yanbian University,2018.(in Chinese)
[25] SCHURCH N J,SCHOFIELD P,GIERLIŃ M,et al.How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?[J].RNA-A Publication of the RNA Society,2016,22(6):839-851.
[26] JANG M K,SON Y,JUNG M H.ATF3 plays a role in adipocyte hypoxia-mediated mitochondria dysfunction in obesity[J].Biochemical and Biophysical Research Communications,2013,431(3):421-427.
[27] MIN K J,KIM C H,SEONG J K,et al.ATF3 inhibits adipocyte differentiation of 3T3-L1 cells[J].Biochemical and Biophysical Research Communications,2012,421(1):38-43.
[28] QI L,SABERI M,ZMUDA E,et al.Adipocyte CREB promotes insulin resistance in obesity[J].Cell Metabolism,2009,9(3):277-286.
[29] PARK H J,KANG Y M,KIM C H,et al.ATF3 negatively regulates adiponectin receptor 1 expression[J].Biochemical and Biophysical Research Communications,2010,400(1):72-77.
[30] ZMUDA E J,QI L M,MIRMIRA R G,et al.The roles of ATF3,an adaptive-response gene,in high-fat-diet-induced diabetes and pancreatic beta-cell dysfunction[J].Molecular Endocrinology,2010,24(7):1423-1433.
[31] HAN B,LIANG W,LIU L,et al.Determination of genetic effects of ATF3 and CDKN1A genes on milk yield and compositions in Chinese Holstein population[J].BMC Genetics,2017,18(1):47.
[32] WU S,HSU L A,CHENG C F,et al.Effect of obesity on the association between ATF3 gene haplotypes and C-reactive protein level in Taiwanese[J].Clinica Chimica Acta,2011,412(11-12):1026-1031.

A Tissue Expression and Polymorphism of ATF3 Gene and Its Association with Meat Quality Traits in Yanbian Yellow Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[5]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[6]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[7]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[8]	YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3428-3437.
[9]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[10]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[11]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[12]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[13]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[14]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[15]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.