干扰MSTN对牛骨骼肌卫星细胞增殖分化的影响

doi:10.16431/j.cnki.1671-7236.2020.02.019

Abstract

Abstract: To explore the effect of myostatin (MSTN) on the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells,the myogenic differentiation model induced by bovine skeletal muscle satellite cells in vitro was used in this study,three interfering RNAs (si-MSTN-1,si-MSTN-2 and si-MSTN-3) were designed and synthesized in the previous stage,si-MSTN-2(si-MSTN) with extremely significant interference effect was transfected into bovine skeletal muscle satellite cells to interfere with MSTN expression.The effect of interfering with MSTN on the proliferation of bovine skeletal muscle satellite cells was detected by EdU staining assay.In vitro myogenic differentiation of bovine skeletal muscle satellite cells transfected with si-MSTN was conducted,the effect of interfering with MSTN on myogenic differentiation of bovine skeletal muscle satellite cells was analyzed by myotube formation and the expression of differentiation markers.The myotube formation during the differentiation of bovine skeletal muscle satellite cells was observed by microscopy.The expression of skeletal muscle satellite cells differentiation markers MyoG and MyHC were detected using Real-time PCR and Western blotting at mRNA and protein levels.The results showed that EdU positive cell ratio in bovine skeletal muscle satellite cells extremely significantly increased after interfering with MSTN (P < 0.01).The number and diameter of myotubes formed in bovine skeletal muscle satellite cells after MSTN interference were extremely significantly higher than that of control group.At mRNA and protein levels,the expression of MyHC,a marker of myogenic differentiation,was extremely significantly increased after MSTN interference (P < 0.01).The results indicated that MSTN interference could significantly promote the proliferation and the process of myogenic differentiation of bovine skeletal muscle satellite cells promote.The results laid a foundation for further studies on the mechanism of MSTN on the myogenic differentiation of bovine skeletal muscle satellite cells.

Key words: MSTN; bovine skeletal muscle satellite cells; proliferation; myogenic differentiation

CLC Number:

S814.8

ZHU Feifei, ZHANG Junxing, ZHANG Linlin, LI Xin, LIU Xinfeng, GUO Yiwen, DING Xiangbin. A Effect of Interfering with MSTN on the Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 479-487.

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A Effect of Interfering with MSTN on the Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells

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