猪circ0015292的鉴定及其在卵泡中的表达研究

doi:10.16431/j.cnki.1671-7236.2019.03.014

Abstract

Abstract:

High throughput sequencing and bioinformatics were used to explore the role of circRNA in porcine follicular development and its possible mechanism,in order to provide the biological foundation for further exploring the regulatory mechanism of circRNA on porcine follicular development.Different levels of follicles and tissue samples from Meishan and Duroc pigs were collected,and then the total RNA was extracted.The differential expression of circRNA in follicles was screened by RNA-Seq technology.Meanwhile,the expression of RNA-Seq in the follicles of Meishan and Duroc pigs was validated and analyzed.At the same time,Real-time PCR was used to make tissue expression profiles.The results confirmed the existence of circ0015292,and it was expressed differently in muscle,heart,liver,spleen,lung,kidney,ovary,fallopian tube,uterus,pituitary gland and hypothalamus,and the expression level was relatively high in heart,spleen,lung and ovary.The expression level of circ0015292 in follicle S of Meishan pigs was extremely significantly higher than that of Duroc pigs (P<0.01),and the expression levels of follicles M1,M2 and L in Duroc pigs were significantly higher than those in Meishan pigs (P<0.05).The potential target genes of circ0015292 (GADD45G,CCR2,IL20RB,CFL1,LAMB3) were related to the development of porcine follicles.The results indicated that circ0015292 was expressed at different levels in different tissues and follicles at different stages;some target genes were involved in cell cycle and ovarian development-related signaling pathways,suggesting that circ0015292 might indirectly participate in follicular development through the regulation of target genes.This result would provide a new idea for the study of pig reproductive mechanism.

Key words: sow; circ0015292; tissue expression profile; RNA-Seq

CLC Number:

S813.3

XIE Su, LI Mengxun, QIU Meiyu, GONG Hongbin, SUN Yishan, SUN Xiaomei, HUANG Tao. Identification of Porcine circ0015292 and Its Expression Pattern in Ovarian Follicles[J]. , 2019, 46(3): 756-763.

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Identification of Porcine circ0015292 and Its Expression Pattern in Ovarian Follicles

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