猪圆环病毒2型和3型混合感染引起的母猪繁殖障碍诊治

doi:10.16431/j.cnki.1671-7236.2022.10.031

Abstract

Abstract: 【Objective】 The purpose of the experiment was to find out the cause of reproductive failure of sows in a pig farm.【Method】 The feed being eaten in the sow feed trough was collect to detect the content of aflatoxin B₁.The oral and nasal swabs,anal swabs and serums of returning sows were collect to detect Porcine circovirus 2 (PCV2),PCV3,Porcine pseudorabies virus (PRV),Porcine parvovirus (PPV),Porcine reproductive and respiratory syndrome virus (PRRSV),Classical swine fever virus (CSFV) by PCR and RT-PCR methods.13 returning sows were randomly dissected,and the spleens,lymph nodes,spinal cords,uterus,ovaries were collected to detect failure virus. ORF2 gene sequence analysis was performed on some positive samples of PCV2 and PCV3. The pathological changes of sows’ ovaries were observed,the results were summarized and analyzed,and the reasons for the low conception rate and continuous estrus return of sows in this farm were diagnosed.【Result】 The content of aflatoxin B₁ in the feed of the pig farm was 4.5-9.0 μg/kg,all within the allowable range of aflatoxin B₁ in feed specified in GB 13078－2017 (＜20 μg/kg).The results of PCR and RT-PCR showed that PCV2,PCV3,PRV,PPV,PRRSV,CSFV in oral and nasal swabs,anal swabs and serums were negative.The positive rate of PCV2 and PCV3 were 69.23% (9/13) and 38.46% (5/13) respectively,and the mixed infection rate of PCV2 and PCV3 was 30.76% (4/13).The results of Real-time quantitative PCR showed that the viral load of PCV2 in spinal cord was the highest,followed by spleen, and the lowest in lymph nodes.The viral load of PCV3 was the highest in spleen,followed by spinal cord,and the lowest in ovary.ORF2 gene sequence analysis showed that PCV2 was subtype 2d and PCV3 was subtype 3a.Ovarian pathological sections showed that the number of follicular cells decreased,and the follicular cavity was irregular,collapsed and flat.【Conclusion】 After diagnosis,it was determined that the main reason for the continuous return to estrus of the sows in this farm was co-infection with PCV2 and PCV3.According to the diagnosis results and the situation of the farm,all of the sows were vaccinated with PCV2 vaccine,meanwhile,the whole farm was disinfected to enhance biosafety prevention.Eventually,the farm achived the normal production.

Key words: Porcine circovirus 2 (PCV2); PCV3; sow; reproductive disorders

CLC Number:

S852.65^＋9.2

SUN Qingshuai, CHEN Yanhong, YAO Lun, SUN Qi, YU Xuexiang, WU Hao, RUAN Shengnan, LEI Mingkai, YAN Weidong, HE Qigai. Diagnosis and Treatment of Reproductive Disorders in Sows Caused by Co-infection of PCV2 and PCV3[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3982-3993.

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Diagnosis and Treatment of Reproductive Disorders in Sows Caused by Co-infection of PCV2 and PCV3

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