PCV3对仔猪生长相关激素及其功能基因表达的影响

doi:10.16431/j.cnki.1671-7236.2022.03.026

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effect of Porcine circovirus (PCV3) on the expression of growth-related hormones and functional genes in piglets, and explore the mechanism of PCV3 causing weight loss in piglets.【Method】 Eight 23 day-old non-infected piglets were randomly divided into PCV3 infected group and DMEM group.Piglets of PCV3 infected and DMEM groups were intramuscular injected with 3 mL of PCV3 virus (10⁹ copies/mL) and 3 mL of DMEM in the neck respectively.The piglets were weighed before injection (0 d) and 3, 7, 14 and 21 d after injection, the clinical manifestations were observed, and serum, oral swab, nasal swab and anal swab were collected.Real-time quantitative PCR method was used to detect the viral load of PCV3, ELISA was performed to determine the content of triiodothyronine (T₃) and thyroxine (T₄) in the serum after infected 3, 7, 14 and 21 d.Real-time quantitative PCR was utilized to determine the relative expression of growth hormone receptor (GHR), insulin-like growth factor type 1 (IGF-1), insulin-like growth factor type 1 receptor (IGF-1R) genes in longissiums dorsi, spleen and liver.【Result】 The final weight and average daily gain of the piglets in infected group were extremely significantly lower than those in DMEM group (P<0.01).The viral load of serum, oral swab, nasal swab and anal swab in infected group reached the highest at 14 d.The content of T₃ in the infected group was extremely significantly higher than that in DMEM group on the 14th and 21st day of infection (P<0.01), and there was no significant difference in the content of T₄ (P>0.05).Compared with DMEM group, the relative expression of IGF-1 gene in longissimus dorsi was extremely significantly decreased (P<0.01), there was no significant difference in the relative expression of GHR, IGF-1 and IGF-1R genes in leg muscle and spleen (P>0.05), the relative expression of GHR gene in liver was extremely significantly increased (P<0.01), and the relative expression of IGF-1R gene was increased significantly (P<0.05).【Conclusion】 PCV3 infection in piglets could cause abnormal metabolism, reduce the growth-promoting and mitogenic effects of IGF-1 on the back muscles, leading to weight loss in piglets.

Key words: Porcine circovirus 3 (PCV3); growth hormone; growth function gene

CLC Number:

S852.65⁺1

SUN Qingshuai, YAN Weidong, LI Chang, WU Hao, LI Changchun, HE Qigai. Effect of PCV3 on the Expression of Growth-related Hormones and Functional Genes in Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1041-1047.

References

[1] 齐梦竹, 李克鑫, 李克钦, 等.猪圆环病毒病的流行病学、临床症状和防治[J].猪业科学, 2020, 37(8):44-47. QI M Z, LI K X, LI K Q, et al.Epidemiology, clinical symptoms and prevention of Porcine circovirus disease[J].Swine Industry Science, 2020, 37(8):44-47.(in Chinese)
[2] 魏恒.猪圆环病毒病的病原特点及防控措施[J].陕西农业科学, 2020, 66(8):94-96. WEI H.The pathogenic characteristics and prevention and control measures of Porcine circovirus disease[J].Shaanxi Journal of Agricultural Sciences, 2020, 66(8):94-96.(in Chinese)
[3] ZHANG H H, HU W Q, LI J Y, et al.Novel circovirus species identified in farmed pigs designated as Porcine circovirus 4, Hunan province, China[J].Transboundary and Emerging Diseases, 2020, 67(3):1057-1061.
[4] WANG Z, CHEN J, WU X, et al.PCV2 targets cGAS to inhibit type Ⅰ interferon induction to promote other DNA virus infection[J].PLoS Pthogens, 2021, 17(9):e1009940.
[5] OPRIESSNIG T, KARUPPANNAN A K, CASTRO A M M G, et al.Porcine circoviruses:Current status, knowledge gaps and challenges[J].Virus Research, 2020, 286:198044.
[6] BERA B C, CHOUDHARY M, ANAND T, et al.Detection and genetic characterization of Porcine circovirus 3 (PCV3) in pigs in India[J].Transboundary and Emerging Diseases, 2020, 67(3):1062-1067.
[7] SAPORITI V, VALLS L, MALDONADO J, et al.Porcine circovirus 3 detection in aborted fetuses and stillborn piglets from swine reproductive failure cases[J].Viruses, 2021, 13(2):264.
[8] CHEN S, ZHANG L, LI X, et al.Recent progress on epidemiology and pathobiology of Porcine circovirus 3[J].Viruses, 2021, 13(10):1944.
[9] YUZHAKOV A G, RAEV S A, ALEKSEEV K P, et al.First detection and full genome sequence of Porcine circovirus type 3 in Russia[J].Virus Genes, 2018, 54(4):608-611.
[10] 贺会利, 李军, 潘艳, 等.广西首例猪圆环病毒3型的发现及其衣壳蛋白序列分析[J].南方农业学报, 2017, 48(8):1499-1503. HE H L, LI J, PAN Y, et al.The first report of Porcine circovirus type 3 infection in Guangxi and sepuence analysis of its capsid protein[J].Journal of Southern Agriculture, 2017, 48(8):1499-1503.(in Chinese)
[11] SUKMAK M, THANANTONG N, POOLPERM P, et al.The retrospective identification and molecular epidemiology of Porcine circovirus type 3 (PCV3) in swine in Thailand from 2006 to 2017[J].Transbound Emerging Diseases, 2019, 66(1):611-616.
[12] 庄金秋, 梅建国, 王艳, 等.猪圆环病毒3型的病原学特征及检测技术研究进展[J].中国动物检疫, 2018, 35(2):59-61. ZHUANG J Q, MEI J G, WANG Y, et al.Research progress on pathogenic charateristics and detection technoques of Porcine circovirus type 3[J].China Animal Health Inspection, 2018, 35(2):59-61.(in Chinese)
[13] 肖兴, 蒋小玲, 芮雪, 等.猪圆环病毒3型感染情况的初步调查[J].猪业科学, 2021, 38(8):73-75. XIAO X, JIANG X L, RUI X, et al.A preliminary invetigation of Porcine circovirus type 3 infection[J].Swine Industry Science, 2021, 38(8):73-75.(in Chinese)
[14] 王雪涛, 林艳, 蔡雨涵, 等.一株猪圆环病毒3型的全基因序列测定及遗传进化分析[J].四川畜牧兽医, 2020, 47(7):23-26. WANG X T, LIN Y, CAI Y H, et al.The complete gene sequence determination and genetic evolution analysis of Porcine circovirus type 3[J].Sichuan Animal and Veterinary Scineces, 2020, 47(7):23-26.(in Chinese)
[15] 李畅, 库旭钢, 王俊伟, 等.猪圆环病毒3型TaqMan荧光定量PCR方法的建立和初步应用[J].畜牧兽医学报, 2018, 49(6):1320-1326. LI C, KU X G, WANG J W, et al.Establishment and preliminary application of TaqMan fluorescent quantitative PCR method for Porcine circovirus type 3[J].Acta Veterinaria et Zootechnica Sinica, 2018, 49(6):1320-1326.(in Chinese)
[16] OUYANG T, NIU G, LIU X, et al.Recent progress on Porcine circovirus type 3[J].Infection, Genetics and Evolution, 2019, 73:227-233.
[17] 汪孟航.PCV3的分子流行病学调查及分离株的致病性研究[D].北京:中国农业科学院, 2021. WANG M H.Molecular epidemiological investigation of PCV3 and pathogenicity of isolates[D].Beijing:Chinese Academy of Agricultural Sciences, 2021.(in Chinese)
[18] HOU L, WANG J, ZHANG W, et al.Dynamic alterations of gut microbiota in Porcine circovirus type 3-infected piglets[J].Frontier Microbiology, 2020, 11:1360.
[19] JIANG H J, WANG D, WANG J, et al.Induction of porcine dermatitis and nephropathy syndrome in piglets by infection with Porcine circovirus type 3[J].Journal of Virology, 2019, 93(4):e02045-18.
[20] SILVA J E, BIANCO S D.Thyroid-adrenergic interactions:Physiological and clinical implications[J].Thyroid, 2008, 18(2):157-165.
[21] GOICHOT B, CARON P, LANDRON F, et al.Clinical presentation of hyperthyroidism in a large representative sample of outpatients in France:Relationships with age, aetiology and hormonal parameters[J].Clinical Endocrinology, 2016, 84(3):445-451.
[22] DEVEREAUS D, TEWELDE S Z.Hyperthyroidism and thyrotoxicosis[J].Emergency Medicine Clinics of North America, 2014, 32(2):277-292.
[23] BOELAERT K, TORLINSKA B, HOLDER R L, et al.Older subjects with hyperthyroidism present with a paucity of symptoms and signs:A large cross-sectional study[J].The Journal of Clinical Endocrinology & Metabolism, 2010, 95(6):2715-2726.
[24] MENCONI F, MARCOCCI C, MARINÓ M.Diagnosis and classification of Graves' disease[J].Autoimmunity Reviews, 2014, 13(4-5):398-402.
[25] PEARCE S H, BRABANT G, DUNTAS L H, et al.013 ETA Guideline:Management of subclinical hypothyroidism[J].European Thyroid Journal, 2013, 2(4):215-228.
[26] GUEVARA-AGUIRRE J, GUEVARA A, PALACIOS I, et al.GH and GHR signaling in human disease[J].Growth Hormone & IGF Research, 2018, 38:34-38.
[27] WERNER H.Tumor suppressors govern insulin-like growth factor signaling pathways:Implications in metabolism and cancer[J].Oncogene, 2012, 31(22):2703-2714.
[28] WERNER H, SARFSTEIN R.Transcriptional and epigenetic control of IGF1R gene expression:Implications in metabolism and cancer[J].Growth Hormone & IGF Research, 2014, 24(4):112-118.
[29] ANDERSEN M.Management of endocrine disease:GH excess:Diagnosis and medical therapy[J].European Journal of Endocrinology, 2014, 170(1):R31-41.
[30] MA Z, DONG A, KONG M, et al.Silencing of the type 1 insulin-like growth factor receptor increases the sensitivity to apoptosis and inhibits invasion in human lung adenocarcinoma A549 cells[J].Cell & Molecular Biology Letters, 2007, 12(4):556-572.

Effect of PCV3 on the Expression of Growth-related Hormones and Functional Genes in Piglets

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