鸡PRR5L基因5'UTR多态性及其与脂肪性状、屠体性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.04.018

Abstract

Abstract:

This study was aimed to investigate the relationship between genetic variations of PRR5L gene with fat traits and carcass traits in chicken.Yellow Dwarf chicken were chosen as research material and primer was designed according to DNA sequence of chicken PRR5L gene (accession No.XM_015287209.1),the single nucleotide polymorphisms(SNP) of PRR5L gene 5'UTR region was detected by PCR direct sequencing.The results showed that there were three polymorphic sites of C480G,G514A and A579G,and all polymorphic sites showed three genotypes.The Chi-square test results showed that the C480G and A579G loci were in Hardy-Weinberg equilibrium (P > 0.05),the G514A locus was not in Hardy-Weinberg equilibrium (P < 0.05).In the fat traits,there were no significant differences among all genotypes of three polymorphic sites of PRR5L gene in abdominal fat weight,abdominal fat rate and intramuscular fat content (P > 0.05).In the carcass traits,there was significant difference between AG and GG genotypes in the G514A locus (P < 0.05).There was also no significant difference of all genotypes in the measured indexes at the C480G and A579G loci (P > 0.05).Therefore,the G514A locus of PRR5L gene 5'UTR might be a site that affected the growing development of chicken breast,however,we needed to further explored whether this site could be used as a marker for the identification of carcass traits in chicken.

Key words: chicken; PRR5L gene; polymorphisms; fat traits; carcass traits

CLC Number:

S813.3

WANG Shunli, CUI Huanxian, LIU Ranran, LI Qinghe, ZHENG Maiqing, WEN Jie, ZHAO Guiping, ZHAO Sumei. Polymorphisms of PRR5L Gene 5'UTR and Its Relationship with Fat and Carcass Traits in Chicken[J]. , 2018, 45(4): 977-983.

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Polymorphisms of PRR5L Gene 5'UTR and Its Relationship with Fat and Carcass Traits in Chicken

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