China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2642-2650.doi: 10.16431/j.cnki.1671-7236.2019.09.018

• Genetics and Breeding • Previous Articles     Next Articles

Association Analysis Between the Polymorphism of SCD1 Gene and Economic Traits in Yanbian Yellow Cattles

PIAO Zhengyu, XIA Guangjun, YAN Changguo, FENG Jian   

  1. Agricultural College of Yanbian University, Yanji 133002, China
  • Received:2018-12-17 Online:2019-09-20 Published:2019-09-21

Abstract: This study was aimed to explore the association between the polymorphism of stearoy-CoA desaturase 1 (SCD1) gene and economic traits in Yanbian Yellow cattle.157 healthy 36-month-old Yanbian Yellow cattle of similar weight were selected,and the blood genomic DNA were extracted from the jugular vein.According to the sequence of bovine SCD1 gene in GenBank (accession No.:AC_000181.1),specific primers were designed by Oligo 6.0 software.The full-length SCD1 gene was amplified by PCR and sequenced directly,the polymorphic was analyzed,and the protein structure was analyzed by bioinformatics software.The carcass traits such as live weight,carcass weight,slaughter rate and backfat thickness were measured before slaughter,the longissimus dorsi muscle was collected and the meat traits such as eye muscle area,cooking loss,drip loss,tenderness,hydraulic power,pH and fatty acid content were measured after slaughter.The correlation between the polymorphism of SCD1 gene and meat quality traits in Yanbian Yellow cattle was analyzed by general linear model analysis.The results showed that there were A→G mutation at 846 bp (g846 A→G) and C→T mutation at 1 022 bp of SCD1 gene (g1022 C→T).g1022 C→T mutation resulted in the mutation of alanie (A) to valine (V),but did not cause tertiary structural changes.Correlation analysis results showed that there was a significant correlation between g846 A→G site and the live weight,carcass weight,drip loss,and the content of stearic acid and linoleic acid (P<0.05),and there was a significant correlation between g1022 C→T site and the live weight,carcass weight and stearic acid content (P<0.05).The results indicated that the two polymorphic sites were significantly correlated with the meat quality traits of Yanbian Yellow cattle,which could be used as a potential molecular marker.

Key words: Yanbian Yellow cattle; SCD1 gene; SNPs; carcass traits; meat quality traits

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