延边黄牛DGATs基因克隆、生物信息学及组织表达分析

doi:10.16431/j.cnki.1671-7236.2021.09.006

Abstract

Abstract: The purpose of this study was to clone the CDS nucleotide sequences of two subtypes (DGAT1 and DGAT2) of diacylglycerol acyltransferase (DGATs) in Yanbian Yellow cattle, analyze the amino acid sequences of two subtypes according to bioinformatics, and explore the expression patterns of two subtypes in Yanbian Yellow cattle. RT-PCR and Real-time quantitative PCR were used to amplify and clone DGATs gene CDS and detect its mRNA expression in 8 tissues of Yanbian Yellow cattle, respectively. BLAST in NCBI was used to analyze its similarity with other species and construct phylogenetic tree. A series of online tools were used to predict the physicochemical properties, primary structure and higher structure of the proteins encoded by it. The results showed that the CDS of DGAT1 and DGAT2 genes were 1 470 and 1 086 bp, coding 489 and 361 amino acids, respectively. Both of them were stable hydrophobic proteins. There were 25 potential phosphorylation sites, 1 N-terminal glycosylation site and 8 transmembrane domains for DGAT1, and 28 potential phosphorylation sites, 2 N-terminal glycosylation sites and 1 transmembrane domain for DGAT2. There was no signal peptide in both of them, so they were not secretory proteins. DGAT1 was dominated by alpha helix and random coil, while DGAT2 was dominated by alpha helix, random coil and extended chain. The expression levels of DGAT1 and DGAT2 genes in small intestine and adipose tissue of Yanbian Yellow cattle were the highest, which were significantly higher than other tissues (P<0.05). The results of this study were of great significance to further study the DGATs gene of Yanbian Yellow cattle and explore its mechanism in the process of fat deposition.

Key words: Yanbian Yellow cattle; DGATs gene; cloning; bioinformatics; expression

CLC Number:

GUO Panpan, JIN Xin, SUN Jianfu, LI Qiang, LI Xiangzi, YAN Changguo. Cloning,Bioinformatics and Tissue Expression Analysis of DGATs Genes in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3158-3170.

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Cloning,Bioinformatics and Tissue Expression Analysis of DGATs Genes in Yanbian Yellow Cattle

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