单宁与饲用纤维素酶对湖羊瘤胃微生物菌群的影响

doi:10.16431/j.cnki.1671-7236.2019.01.013

Abstract

Abstract:

The purpose of this study was to investigate the effects of adding tannins and feeding cellulase on rumen microbes in the growth and fattening period of Hu sheep using high-throughput sequencing technology,and provide useful data for the better utilization of tannins and cellulase in ruminant production.Thirty-six meat Hu sheep with good growth and average body weight (19.85±1.45) kg at 3 months old were randomly divided into control group and 3 treatment groups.The control group was fed with basal diet,and three treatment groups were added 0.1% tannin (tannin group),0.1% feeding cellulase (cellulase group) and 0.1% tannin +0.1% feeding cellulase (mixed group) in the basal diet respectively,3 replicates per group,each repeat 3 sheep.The test period was 70 days,in which the transition period was 7 days,the pre-test period was 7 days,and the positive feeding period was 56 days.At the end of the experiment,the rumen fluid of Hu sheep was collected,and the total DNA of the bacteria was extracted and PCR amplification was conducted.The amplified products were sequenced by Illumina Hi Seq 2500 for high-throughput sequencing.The results showed that:① A total of 957 440 pairs of microbial samples were obtained from 12 rumen samples,with an average of 55 997 clean tags,after filtering chimeras,a total of 502 965 effective tags were generated.AvgLen of rumen microbial samples from each group of sheep were between 419 and 420;②The Ace index and Chao1 index of rumen microorganisms in each group had no significant difference (P > 0.05);Compared with the control group,the Shannon index of rumen microorganisms in tannin,cellulose and mixed groups were significantly increased (P < 0.05).The Simpson index of rumen microorganisms in cellulase group was significantly lower than that in the control group (P < 0.05).③At the level of phylum:The abundance of Bacteroidetes of tannin group was significantly lower than that of the control group (P < 0.05);The abundance of the Firmicutes in tannin,cellulose and mixed group were lower than that of the control group,but the difference was not significant (P > 0.05).④On the genus level,the abundance of Prevotella_1 in tannin group was droped,and there was significant difference compared with the control and cellulase groups (P < 0.05),but there was no significant difference with mixed groups (P > 0.05).The abundance of Rumen-bacterium in tannin and cellulase groups were higher than that of the control and mixed group,and the difference with mixed group was significant (P < 0.05).In conclusion,adding tannin and cellulase at the same time could improve the diversity and abundance of rumen flora,affect the structure of rumen flora,and alleviate the inhibition of tannin on Bacteroidetes and Firmicutes.Mixed addition could alleviate the inhibition of cellulose decomposition by adding tannin alone.The dominant bacteria in the rumen of each group of Hu sheep were Bacteroidetes and Firmicutes.On the genus level,the dominant bacteria in the rumen of each group were studied Rikenellaceae_RC9_gut_group,Bacterium,Prevotella_1 and Rumen_bacterium.

Key words: tannin; feeding cellulase; Hu sheep; microbial flora; high-throughput sequencing

CLC Number:

S816.7

ZHAO Mengdi, DI Lingfeng, TANG Zeyu, CAO Xue, LI Chengyun. Effects of Tannin and Feeding Cellulase on Rumen Microflora of Hu Sheep[J]. , 2019, 46(1): 112-122.

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Effects of Tannin and Feeding Cellulase on Rumen Microflora of Hu Sheep

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