鸭肠炎病毒感染鸭肝脏组织miRNA表达谱差异分析

doi:10.16431/j.cnki.1671-7236.2021.11.027

Abstract

Abstract: In order to explore the differential expression of miRNA in liver of duck infected by Duck enteritis virus (DEV), in this experiment, 30-day-old hemp ducks were inoculated with the DEV-GZ strain through the leg muscles. Duck liver tissue samples were collected at 66, 90 and 114 h after infection, and total RNA was extracted from the tissue. After passing the quality inspection, high-throughput sequencing technology performed miRNA sequencing on the samples of control and test groups, and screened out the differentially expressed miRNA in liver of DEV-infected duck, performed bioinformatics GO functional classification and KEGG signal pathway analysis, and randomly selected some differentially expressed miRNA for Real-time quantitative PCR verification. The results showed that when DEV infected ducks at 66, 90 and 114 h, the number of differentially expressed miRNAs in liver were 227, 225 and 231, respectively. GO function annotations showed that the differentially expressed miRNA in liver of infected ducks were mainly classified into cellular processes, single-organism processes and metabolic processes in the classification of biological processes. In the classification of cell components, they were mainly classified into cells, cell parts and organelles. In molecular functions, the classification mainly included binding molecular function and catalytic activity function category. KEGG pathway enrichment showed that differentially expressed miRNA mainly involved PI3K-Akt, JAK-STAT, phosphatidylinositol signaling pathway system, ECM-receptor interaction, MAPK, Wnt, Toll-like receptors, IL-17, lipid metabolism, calcium signal pathway, cAMP and other signaling pathways. Among them, differentially expressed miRNA mainly played a role in the biological system and nervous system after 66 h of infection, mainly played a role in endocrine system and digestive system after 90 h of infection, and mainly played a role in the systemic biology, immune and digestive system after 114 h of infection. 10 differentially expressed miRNAs were selected for Real-time quantitative PCR verification, and the results were consistent with the results of high-throughput sequencing. It indicated that DEV infection had a significant impact on the expression of miRNA in liver of duck, and provided a reference for revealing the pathogenic mechanism of DEV from the perspective of host miRNA.

Key words: Duck enteritis virus (DEV); high-throughput sequencing; miRNA; differential expression

CLC Number:

S852.65⁺7

ZENG Maoqin, LIU Yanhan, ZHANG Qiandong, BI Wenwen, YE Ni, WANG Yizhou, YANG Ying, CHENG Zhentao, WEN Ming. Differential Analysis of miRNA Expression Profiles in Liver of Duck Infected with Duck Enteritis Virus[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4170-4181.

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Differential Analysis of miRNA Expression Profiles in Liver of Duck Infected with Duck Enteritis Virus

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