产肠毒素大肠杆菌F41感染猪小肠上皮细胞初期lncRNA的表达谱分析

doi:10.16431/j.cnki.1671-7236.2021.05.022

Abstract

Abstract: To explore the role of long noncoding RNA (lncRNA) at early stage of enterotoxigenic Escherichia coli (ETEC) infection,the expression profile of lncRNAs in the porcine intestinal epithelial cells (IPEC-J2) infected with ETEC was analyzed.ETEC F41 strain was used to infect IPEC-J2 cells.The cells were collected before infection and at early infection (4 h post infection),followed by high-throughput sequencing via the Illumina HiSeq Xten platform.A total of 9 975 lncRNAs were discovered in this study.Compared with cells before infection,100 differentially expressed lncRNAs were found at the early stage of ETEC infection.Among them,40 differentially expressed lncRNAs were up-regulated,and 60 differentially expressed lncRNAs were down-regulated.The target genes of differentially expressed lncRNAs were predicted using miRanda-3.3a and psRobot_v1.2 software,followed by GO and KEGG pathway enrichment analysis.The results showed that at the early stage of infection,the target genes of differentially expressed lncRNAs were significantly enriched in the GO functional items such as nuclear,nucleolus,metabolic process regulation and development process.The KEGG analysis showed that the target genes of differentially expressed lncRNAs at the early stage of infection were significantly enriched in the pathways including PI3K-Akt signaling pathway,regulation of actin cytoskeleton,focal adhesion and cell cycle.Real-time quantitative PCR was performed to validate the expression of 5 randomly selected differentially expressed lncRNAs,which showed that the expression differences before and after infection were consistent with the sequencing results.This study analyzed the lncRNA expression profile of IPEC-J2 cells infected with ETEC at the early stage,which provided a basis for exploring the functional mechanism of key lncRNAs during ETEC infection.

Key words: ETEC; lncRNA; high-throughput sequencing; pig

CLC Number:

S858.28

LI Pei, LIU Guomei, LIU Yingying, LIU Mandi, LI Yan, FAN Jinghui. Analysis of lncRNA Expression Profile in Porcine Intestinal Epithelial Cells Infected with Enterotoxigenic Escherichia coli F41 at Early Stage[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1707-1716.

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Analysis of lncRNA Expression Profile in Porcine Intestinal Epithelial Cells Infected with Enterotoxigenic Escherichia coli F41 at Early Stage

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