石河子地区宠物犬蜱的种类鉴定及其携带布鲁氏菌的检测

doi:10.16431/j.cnki.1671-7236.2021.05.023

Abstract

Abstract: To understand the species of ticks from pet dogs and their carrying status of Brucella in Shihezi,Xinjiang,on the basis of morphological classification,the ticks from pet dogs were detected by molecular biology method based on mitochondrial gene 12S rRNA and cytochrome oxidase Ⅰ (COⅠ).DNAMAN software was used to compare and analyze sequence homology,and sequence analysis software Mega 7.0 was used to construct a phylogenetic tree of tick species by using the Neighbor Joining method to analyze the genetic evolutionary relationship of tick species.Based on the Brucella outer membrane protein Omp22 gene,ticks from pet dogs were tested for Brucella by PCR to determine the Brucella carrying status of ticks from pet dogs.The results showed that the morphology of 436 ticks from pet dogs were consistent with the molecular biology identification results of mitochondrial genes (12S rRNA and COⅠ),and they were all Rhipicephalus turanicus,one of the dominant species in Xinjiang.The phylogenetic tree of tick species constructed based on the 12S rRNA gene showed that the sequence of Rhipicephalus turanicus from pet dogs obtained in this experiment was clustered well with the sequence of the corresponding tick known in GenBank.The results of nested PCR amplification based on the Omp22 gene of Brucella showed that the positive rate of Brucella carried by Rhipicephalus turanicus from pet dogs was 4.82% (21/436),and the homology was 100%.BLAST comparison showed that Brucella carried by Rhipicephalus turanicus from pet dogs obtained in this experiment was related to the Xinjiang epidemic strain YC31 (GenBank accession No.:MK201679.1),QH5 (GenBank accession No.:MK201678.1),EM3 (GenBank accession No.:MK201677.1) and ML9 (GenBank accession No.:MK201676.1),and the homologies were all 100%.In this study,the species of ticks from pet dogs in Shihezi,Xinjiang,and their carrying Brucella were studied by morphology and molecular biology,and provided basic information for the species of parasitic ticks on pet dogs and the monitoring and control of tick-borne diseases.

Key words: pet dog; Rhipicephalus turanicus; molecular identification; Brucella

CLC Number:

S852.7

SONG Shengnan, GUO Jia, YANG Yicheng, SANG Chunli, YANG Meihua, WANG Yuanzhi. Species Identification of Ticks from Pet Dog and Detection of Brucella Carried by Ticks in Shihezi Area[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1717-1724.

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Species Identification of Ticks from Pet Dog and Detection of Brucella Carried by Ticks in Shihezi Area

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