乌湖杂交羊GnRHR与INHA基因多态性及其互作效应对产羔数的影响

doi:10.16431/j.cnki.1671-7236.2020.03.016

Abstract

Abstract: This study was aimed to analyze the influence of genetic variation of GnRHR and INHA genes and its interaction effect on the number of lambing in sheep.Primers were designed according to DNA sequence of GnRHR and INHA genes (GenBank accession No.:AH004943 and U16237).PCR-SSCP was used to detect the genetic polymorphisms of GnRHR and INHA genes in Wuhu sheep,Hu sheep and Black bone sheep,the correlation between polymorphic loci and litter size and the interaction effect between GnRHR and INHA genes were analyzed.The results showed that AA,AB and BB genotypes,GG,GC and CC genotypes were detected in GnRHR and INHA genes of Wuhu sheep and Hu sheep,no polymorphism site was found in Black bone sheep with few samples.G→C mutation occurred at 198 bp of GnRHR gene CDS,resulting in glycine to arginine,which was missense mutation;T→C mutation occurred at 877 bp of INHA gene,which was synonymous mutation (still aspartic acid).Genetic analysis results showed that GG and AA were dominant genotypes,and G and A were dominant alleles.The Chi-square test results showed that GnRHR and INHA genotypes distribution was in Hardy-Weinberg equilibrium (P>0.05).By analyzing the interaction effect between GnRHR and INHA genes,it was found that there was a significant difference in the interaction effect between Wuhu and Hu sheep (P<0.05).The interaction effect of ABCC genotype was the largest,and AAGG genotype was the smallest.The average lambing number of ABCC genotype were 1.68 and 1.32 in Wuhu sheep and Hu sheep,respectively,which was higher than that of AAGG genotype,and the lambing number of ABCC genotype was higher than that of AB and CC genotypes,which indicated that the interaction effect was positively correlated with lambing number.This results suggested that interaction genotype of INHA (T877C) and GnRHR (G198C) was closely related to the lambing number of sheep,which had a significant effect on the litter size in sheep.

Key words: Wuhu sheep; GnRHR gene; INHA gene; PCR-SSCP; litter size trait; interactive effect

CLC Number:

S813.3

LIU Haixia, HAN Dayong, ZHU Aiwen, YAN Wei, WANG Buzhong, CAI Zijian. Effect of the Polymorphism of GnRHR and INHA Genes and Its Interaction on Litter Size in Wuhu Hybrid Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 780-788.

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Effect of the Polymorphism of GnRHR and INHA Genes and Its Interaction on Litter Size in Wuhu Hybrid Sheep

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