中国美利奴羊TLR2基因多态性及其与布鲁氏菌病的相关性分析

doi:10.16431/j.cnki.1671-7236.2018.05.003

Abstract

Abstract:

This experiment was aimed to study the correlation between Toll-like receptor 2 (TLR2) gene polymorphisms and susceptibility to brucellosis of China Merino sheep.Bioinformatics methods were used for comparing the TLR2 gene sequences of sheep which were published on NCBI,and selected abundant fragments of polymorphic loci for amplification.The method of PCR-SSCP was used to detect the polymorphism of TLR2 gene from 206 brucellosis negative samples and 80 brucellosis positive samples of Chinese Merino sheep,the PCR products of different alleles were sequenced to determine the polymorphic loci of TLR2 gene.The correlation between allele frequency,genotype frequency and polymorphism of each SNP and susceptibility to brucellosis were analyzed by Chi square test.Bioinformatics analysis software was used to analyze the secondary structures of RNA and protein.The results showed that 3 SNPs (C1731T,G1737C and G1749T) were detected in the sequence of 279 bp,which didn't lead to the change of corresponding amino acids,and belonged to nonsense mutation.There was no significant difference in allele frequency and genotype frequency among the cases and controls (P>0.05).The secondary structures and the minimum free energy of RNA were changed at each mutation site,but the secondary structures of protein was not changed.In conclution,there was no correlation between 3 SNPs (C1731T,G1737C and G1749T) of TLR2 gene and brucellosis susceptibility of Chinese Merino sheep.

Key words: China Merino sheep; TLR2 gene; PCR-SSCP; polymorphism; brucellosis

CLC Number:

Q813.3

LUO Cheng, WANG Yuanyuan, WANG Huimin, TAN Jun, ZHOU Guangpu, GAO Jianfeng. Polymorphism of TLR2 Gene in Chinese Merino Sheep and Its Correlation Analysis with Brucellosis[J]. , 2018, 45(5): 1145-1152.

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Polymorphism of TLR2 Gene in Chinese Merino Sheep and Its Correlation Analysis with Brucellosis

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