猪Leptin基因多态性及其与产仔性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.04.026

Abstract

Abstract:

The research was aimed to analyze the association of Leptingene and breed traits on pigs. Three pairs of primers were designed which based on DNA sequence of pig Leptin gene (GenBank accession No.U66254, AF026976),the single nucleotide polymorphisms (SNPs) of Leptin gene were detected by PCR-SSCP in Sujiang and Jiangquhai pigs. The results showed that three genotypes of AA,AB and BB were identified, and the mutation (T3469C) in CDS of Leptin gene was found,but amino acid change weren't happened. The Chi-square test results showed that the Leptin genotype distribution of Sujiang and Jiangquhai pigs was in Hardy-Weinberg equilibrium (P>0.05). The polymorphism information content (PIC) were moderate polymorphism in Sujiang pigs,and low polymorphism in Jiangquhai pigs. The statistic analysis results showed that there were no significant association between the SNP of Leptin genotype and litter size trait(P>0.05). But the average litter size of T3469C site AB genotype was higher than AA and BB genotypes, it was concluded that the T3469C polymorphism of Leptingene might be the genetic marker which potentially affected pig breed traits.

Key words: Sujiang pigs; Jiangquhai pigs; Leptin gene; PCR-SSCP; litter size trait

CLC Number:

S814.8

ZHU Ai-wen, ZHOU Chun-bao, NI Li-gang, HAN Da-yong. The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs[J]. , 2017, 44(4): 1122-1127.

References

[1] 刘华珍,彭克美. Leplin及其受体的研究进展[J].华中农业大学学报,2003,22(1):90-94.
[2] Zhang Y,Proenca R,Maffei M,et al. Positional coloning of the mouse obese gene and its human homologue[J].Nature,1994,372(6505):425-432.
[3] Neuenschwander S,Rettenberger G,Meijerink E,et al. Partial characterization of porcine obesity gene(OBS) and its localization to chromosome 18 by somatic cell hybrids[J]. Anim Genet,1996,26(4):265-268.
[4] Bidwell C A,Ji S,Frank G R,et al. Cloning and expression of the porcine obese gene[J]. Anim Biotech,1997,8(2):191-206.
[5] 戴茹娟,李宁. 猪肥胖基因(ob)位点的RFLP多态性分析[J].农业生物技术学报,2000,8(2):123-124.
[6] 严燕. 安徽四个地方猪种Leptin基因多态性研究及其与产仔性状的关联分析[D]. 合肥:安徽农业大学,2009.
[7] Kennes Y M,Murphy B D,Pothier F,et al. Characterization of swine leptin ploymorphisms and their associstion with production traits[J]. Anim Genet,2001,32(4):215-218.
[8] 萨姆布鲁克J,弗里奇E F,曼尼阿蒂斯. 分子克隆实验指南[M]. 北京:科学出版社,1999.
[9] 张冰,杨公社,孙超,等. LEP基因对陆川猪和大白猪产仔数的影响[J].畜牧兽医学报,2007,38(6):608-613.
[10] 寿奎均,孔伟林,陈成红,等. Leptin与动物繁殖[J].中国畜禽种业,2015,2:69-71.
[11] 王春萍,刘一帆,田林红,等. 瘦素与性激素水平在代谢综合征儿童青春期中的变化规律及相互关系[J].中国妇幼保健,2014,29(35):5871-5874.
[12] 花伦,卓勇,徐盛玉,等.瘦素对哺乳动物初情启动的影响及其可能机制[J]. 中国畜牧杂志,2016,52(13):91-94.
[13] 张谊. 瘦素与哺乳动物繁殖活动关系的研究进展[J]. 中国畜牧兽医,2015,42(7):1836-1841.
[14] 孙琦,段昭颖,堪宁,等. 瘦素对生殖调控作用的研究进展[J]. 南昌大学学报(医学版),2013,8:85-87.
[15] 赵鸿昊,李学华,曾聪. Leptin基因在团头鲂成鱼各组织和早期发育中的表达分析[J]. 华中农业大学学报,2016,35(1):92-98.
[16] 黄艳娜,何少婧,李莉,等. 陆川猪瘦素基因的克隆及序列分析[J]. 黑龙江畜牧兽医(科技版),2015,11:92-95.
[17] 冯岩,闰红,李贵霞,等. Leptin、IGF-1、IGFBP-3、LH峰值与性早熟关系的研究进展[J]. 河北医药,2014,36(20):3161-3164.
[18] Jeffrey M F,Jeffrey L H. LEP and the regulation of body weight in mammals[J].Nature,1998,395:763-770.
[19] 刘德武,杜颖军,张豪,等. 猪Leptin基因的SNP筛查及其与生长性状的关联分析[J].华中农业大学学报,2008,27(2):251-257.
[20] 康永刚,李明艳. Leptin基因第3469位点基因型对合作猪繁殖性能和体尺的影响[J]. 畜牧与兽医,2015,47(10):69-72.
[21] 朱世平,刘颖,董文华,等.大白猪Leptin基因外显子3多态性及其与部分经济性状的关系[J]. 中国畜牧杂志,2014,50(17):5-8.
[22] Jang Z H,Gibson J P. Genetic polymorphisms in theLeptin gene and their association with fatness in four pig breeds[J].Mamm Genome,1999,10(2):191-193.
[23] Chen C C,Chang T,Su H Y. Genetic polymorphisms in porcine Leptin gene and their association with reproduction and production traits[J].Aust J Agr Res,2004,55(6):699-704.

The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs

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