感染肺炎支原体姜曲海猪肺组织差异表达circRNAs的筛选与分析

doi:10.16431/j.cnki.1671-7236.2021.06.035

Abstract

Abstract: In order to investigate the differential expression profile of circular RNA (circRNA) in the lung tissue of pigs infected with Mycoplasma hyopneumoniae (Mhp) as well as its role in Mhp infection.Jiangquhai pigs were used as subjects in this experiment,which were divided into Mhp-infected and control groups.On the 28th day post inoculation,the lung tissues were collected from all of the experimental pigs for analysis of circRNA expression by high-throughput sequencing and bioinformatics software.Data comparison showed that a total of 23 632 circRNAs were identified,with 213 differentially expressed circRNAs(DEcircRNAs).Among them,97 up-regulated and 116 down-regulated.Four from 213 DEcircRNAs were randomly selected and verified by Real-time quantitative PCR,and the results were consistent with those obtained by sequencing.Host genes of DEcircRNAs were annotated to immune-related signaling pathways,including antigen processing and presentation,lysosome and leukocyte transendothelial migration.The target relationship analysis of circRNA-miRNA-mRNA showed that three DEcircRNAs with the highest number of sponge miRNAs were circRNA-17284 (21),circRNA-04848 (19) and circRNA-17270 (19),of which 6 targeted miRNAs related to immune regulation were predicted,including ssc-miR-4331,ssc-miR-370,ssc-miR-328,ssc-miR-30c-3p,ssc-miR-122 and ssc-miR-125b.In this study,the expression profile of circRNA in the lung tissue of pigs infected with Mhp was determined,and the DEcircRNAs related to immune regulation was screened,which helped to clarify the mechanism of susceptibility of Jiangquhai pigs to Mhp infection,and provide scientific basis for disease-resistant breeding research.

Key words: Jiangquhai pigs; Mycoplasma hyopneumoniae; circRNA; high throughput sequencing

CLC Number:

Q786

NI Ligang, ZHAO Xuting, WANG Xiaoyan, XU Pan, GAN Yuan, JIN Wen. Screening and Analysis of Differentially Expressed circRNAs in the Lung Tissue of Jiangquhai pigs Infected with Mycoplasma hyopneumoniae[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2212-2220.

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Screening and Analysis of Differentially Expressed circRNAs in the Lung Tissue of Jiangquhai pigs Infected with Mycoplasma hyopneumoniae

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