猪miR-199a-5p对脂质生成的影响及其靶基因的预测和分析

doi:10.16431/j.cnki.1671-7236.2021.01.006

Abstract

Abstract: The aim of this study was to analyze the effect and mechanism of miR-199a-5p on lipid production of porcine intramuscular adipocytes.The longissimus dorsi muscle and subcutaneous fat tissue of Huainan pigs were obtained at different fattening periods (before,middle and after fattening periods),and the expressional trend of miR-199a-5p was detected by Real-time quantitative PCR.mimics of miR-199a-5p was synthesized and transfected in porcine intramuscular adipocytes,the lipid deposition was detected by Oil Red O staining after differentiation.The relationship between the previously detected meat quality-related differently expressed mRNAs,lncRNAs,circRNAs and miR-199a-5p was analyzed by miRanda software,and the function of miR-199a-5p target genes (target mRNAs,co-expressed mRNAs of lncRNAs,parent genes of circRNAs) was analyzed by GO and KEGG enrichment.The results indicated that miR-199a-5p was continuously upregulated in longissimus dorsi muscle during fattening period,but it was firstly upregulated and then downregulated in subcutaneous adipose tissue.Overexpression of miR-199a-5p inhibited adipogenesis in intramuscular adipocytes.There were 9 mRNAs,5 lncRNAs,and 1 258 circRNAs owned miR-199a-5p binding sites,KEGG analysis showed that these genes were mainly enriched in sugar,lipid and protein metabolism,and GO enrichment mainly involved plasma membrane region and acting binding.The results indicated that miR-199a-5p might participate in muscle development,adipogenesis and lipid metabolism by interaction with lncRNAs and circRNAs,and it could be used as a candidate miRNA for meat quality trait.

Key words: miR-199a-5p; adipogenesis; lncRNAs; circRNAs

CLC Number:

XING Baosong, WANG Jing, CHEN Junfeng, REN Qiaoling, MA Qiang, GUO Hongxia, PAN Chuanying, CAO Hai. Effect of miR-199a-5p on Adipogenesis and Prediction and Analysis of Its Target Genes[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 55-63.

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Effect of miR-199a-5p on Adipogenesis and Prediction and Analysis of Its Target Genes

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