葛根素对Akt通路调控3T3-L1脂肪细胞分化的影响

doi:10.16431/j.cnki.1671-7236.2018.01.007

Abstract

Abstract:

In order to study the effect of puerarin on differentiation of 3T3-L1 pre-adipocytes, explore the potential mechanism of puerarin in adipogenic differentiation. Puerarin was added into the medium to induce differentiation with final concentration of 0,10 and 50 μmol/L in the process of adipogenesis. The effects of puerarin on accumulation of lipid droplets and the content of triglyceride were measured by oil red O staining and triglyceride assay kit in 3T3-L1 adipocytes, respectively. The mRNA expression of CCAAT-enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) in adipocytes were detected by quantitative Real-time PCR, and the protein expression levels of adipogenic transcription factor and Akt signaling pathway were qualified by Western blotting. The results showed that 10 μmol/L puerarin extremely significantly increased the accumulation of lipid droplets and triacylglycerol (TG) in mature adipocytes, which extremely significantly promoted the mRNA and protein expression levels of adipogenic transcription factor C/EBPα and PPARγ (P<0.01). Further studies showed that stimulation of puerarin enhanced the phosphorylation level of the Akt signaling pathway Ser473 protein compared to control group, suggested that the phosphorylation of Akt signaling pathway played a major role in promoting adipogenic differentiation. In conclusion, puerarin could promote the differentiation of 3T3-L1 pre-adipocytes and improve the sensitivity of insulin, and its mechanism was related to the phosphorylation level of Ser473, which activated Akt signaling pathway. This research would provide a new insights into the mechanism of insulin and a new ideas for treatment of insulin resistance related diseases.

Key words: puerarin; adipogenesis; 3T3-L1; Akt pathway

CLC Number:

Q813.1⁺1

YUN Jinyan, YU Yongsheng, CAO Yang, ZHANG Lichun, JIN Haiguo, LUO Xiaotong, WEI Tian, LI Xin, JIN Xin. Effect of Puerarin on Regulation Adipocytes Differentiation Through Akt Pathway in 3T3-L1 Cells[J]. , 2018, 45(1): 57-64.

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Effect of Puerarin on Regulation Adipocytes Differentiation Through Akt Pathway in 3T3-L1 Cells

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