不同浓度油酸对延边牛骨骼肌卫星细胞成脂转分化的影响

doi:10.16431/j.cnki.1671-7236.2021.04.005

Abstract

Abstract: The present study was aimed to explore the effect of oleic acid on the adipogenic transdifferentiation of Yanbian bovine skeletal muscle satellite cells.The experiment was set up 1 blank control group (CON) and 3 oleic acid (OA) induction groups:50,100 and 200 μmol/L oleic acid groups (OAL,OAM and OAH).96 h after oleic acid induction,the effect of oleic acid on cells was evaluated by measuring cell size and viability.Oil red O staining method and triglyceride determination were used to verify the formation of lipid droplets,the expressions of related myogenic adipogenic genes were measured by Real-time quantitative PCR to verify the production of adipocytes.The results showed that,compared with the control group,when oleic acid was added,lipid droplets were formed in Yanbian cattle skeletal muscle satellite cells,and the amount of lipid droplet formation and trigbyceride accumulation were dose-dependent with oleic acid.Real-time PCR measurement results showed that the myogenic related factors Pax3 and MyoD were significantly down-regulated (P<0.05),the adipogenic factors C/EBPβ and PPARγ were significantly up-regulated (P<0.05),the fatty acid metabolism-related factor SCD was significantly down-regulated (P<0.05),and the PLIN2 gene was significantly up-regulated (P<0.05).The results of the experiment showed that the induction and treatment of Yanbian bovine skeletal muscle satellite cells with oleic acid could promote the adipogenic transdifferentiation of the cells.

Key words: oleic acid; Yanbian cattle; skeletal muscle satellite cells; adipogenesis

CLC Number:

S823

SUN Jianfu, ZHANG Junfang, SUN Bin, WANG Ying, CUI Yan, LI Qiang, CHOI Seong H, SHIN Jong S, LI Xiangzi. Effects of Different Concentrations of Oleic Acid on Adipogenic Transdifferentiation of Yanbian Bovine Skeletal Muscle Satellite Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1188-1195.

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Effects of Different Concentrations of Oleic Acid on Adipogenic Transdifferentiation of Yanbian Bovine Skeletal Muscle Satellite Cells

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