添加环格列酮对延边黄牛骨骼肌卫星细胞成脂转分化的影响

doi:10.16431/j.cnki.1671-7236.2020.03.008

Abstract

Abstract: This study was aimed to investigate the effect of ciglitazone,a peroxisome proliferator-activated receptor (PPARγ) agonist,on the adipogenic transdifferentiation of skeletal muscle satellite cells in Yanbian Yellow cattle.The skeletal muscle satellite cells were treated with ciglitazone at concentrations of 0 (CON group),5 (CL group),10 (CM group) and 20 (CH group) μmol/L for 96 h.The formation of lipid droplets was evaluated by oil red O staining and triglyceride amounts was determined by triglyceride determination kit.Real-time quantitative PCR and Western blotting were employed to detect whether adipocytogenesis related factors were involved.The results showed that the area of oil red O and the amounts of triglyceride were both significantly increased after treated with ciglitazone compared with control group (P<0.05).Moreover,the area of oil red O and the amounts of triglyceride were positively correlated with the concentration of ciglitazone.Compared with control group,the mRNA and protein expression of adipocytic transcription factors,including PPARγ,CCAAT/enhancer binding protein alpha (C/EBPα) and peripoietin-2 (PLIN2),were significantly upregulated in ciglitazone addition groups (P<0.05).However,the expression of myogenic-related factor MyoD was downregulated after treated with ciglitazone (P<0.05).The results indicated that ciglitazone could promote the transdifferentiation of skeletal muscle satellite cells to adipocytes in cattle.

Key words: Yanbian Yellow cattle; skeletal muscle satellite cells; lipogenesis; PPARγ; ciglitazone

CLC Number:

Q952.6

YAN Yan, ZHANG Junfang, SUN Bin, WANG Ying, SUN Jianfu, SUN Xiaojiao, CUI Yan, JIN Xin, YAN Changguo, LI Xiangzi. Effect of Ciglitazone on Adipogenic Transdifferentiation of Skeletal Muscle Satellite Cells in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 714-721.

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Effect of Ciglitazone on Adipogenic Transdifferentiation of Skeletal Muscle Satellite Cells in Yanbian Yellow Cattle

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