延边黄牛脂蛋白脂肪酶基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.029

Abstract

Abstract: This experiment was conducted to explore the association between the polymorphism of lipoprteinlipase (LPL) gene and meat quality traits in Yanbian Yellow cattle.In this study,longissimus dorsi muscle of 80 healthy Yanbian Yellow cattle aged 30 months were used as experimental materials.The SNP of LPL gene was detected by PCR sequencing and HRM,respectively.Genetic effects of the experimental population was analyzed and correlated with the meat quality data of Yanbian Yellow cattle.The PCR sequencing results showed that there were two polymorphic loci (g.6215 A>G and g.18341 C>T) of LPL gene in Yanbian Yellow cattle.g.6215 A>G of LPL gene showed two genotypes of AA and AG,and the dominant allele was A;g.18341 C>T showed two genotypes of CC and CT,C was the dominant allele.The detection results of two loci in the reference showed that g.355427 T>A showed two genotypes of AA and AT,A was the dominant allele,and c.322 G>A,showed no genotyping.All of SNPs showed moderate polymorphism (0.25<PIC<0.5) and were in Hardy-Weinberg equilibrium.The results of HRM genotyping showed that there were three SNPs in genotyping,which were correlated with meat quality traits of Yanbian Yellow cattle.g.6215 A>G was significantly correlated with carcass weight,fat content,protein content and water content of Yanbian Yellow cattle,which were:carcass weight,fat content of AG genotype were significantly higher than AA genotype (P<0.05),and protein content,water content of AA genotype were significantly higher than AG genotype (P<0.05).g.18341 C>T was significantly correlated with pre-slaughter weight,carcass weight and backfat thickness of Yanbian Yellow cattle,which showed that CT genotype was significantly higher than CC genotype (P<0.05).In g.355427 T>A,pre-slaughter weight,backfat thickness and fat content of AT genotype were higher than AA genotype (P<0.05).To sum up,g.6215 A>G,g.18341 C>T and g.355427 T>A had an effect on the meat quality of Yanbian Yellow cattle,and LPL gene could be used as the dominant candidate gene and effective molecular marker for breeding and improvement of Yanbian Yellow cattle breeds.

Key words: Yanbian Yellow cattle; LPL gene; meat quality traits; SNP; genotyping

CLC Number:

S813.3

ZHANG Jiasu, GUO Siwei, ZHANG Xinxin, ZHANG Congcong, ZHANG Shuai, YIN Baozhen, ZHANG Luomeng, XIA Guangjun. Genetic Polymorphism of LPL gene and Its Correlation Analysis of Meat Quality Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2530-2538.

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Genetic Polymorphism of LPL gene and Its Correlation Analysis of Meat Quality Traits in Yanbian Yellow Cattle

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