褪黑素对荷斯坦奶牛妊娠率及生殖激素的影响

doi:10.16431/j.cnki.1671-7236.2021.07.028

Abstract

Abstract: The purpose of this study was to investigate the effects of melatonin (MT) on the pregnancy rate and serum reproductive hormones in Holstein dairy cows.A total of 150 first mating Holstein dairy cows with natural estrus were determined by pedometer method.Among them,70 cows were injected with 30 mg melatonin subcutaneously in the neck,and artificial insemination was performed 12 h later.170 Holstein dairy cows were selected for the first time of postpartum mating for the treatment of simultaneous ovulation and timing insemination.Among them,90 Holstein dairy cows were injected with gonadotropin-releasing hormone (GnRH) and 30 mg melatonin subcutaneously and intramuscularly at the same time.Artificial insemination was carried out 16 h later.153 Holstein dairy cows were selected for subcutaneous injection of melatonin.Pregnancy examination was carried out 20~35 d after insemination.The number of cows in the first mating and second mating and the number of calves were recorded in detail.25 Holstein dairy cows with simultaneous ovulation and timing insemination were selected,8 h after subcutaneous injection of melatonin,the contents of melatonin,follicle stimulating hormone (FSH),luteinizing hormone (LH) and estradiol (E₂) in the serum were detected by radioimmunoassay,and the content of progesterone (P₄) in the serum of pregnant cows at 35 d was detected.The results showed that compared with control group of natural estrus and synchronous ovulation,the pregnancy rate and calving rate of Holstein dairy cow of the corresponding subcutaneous injection of melatonin group were significantly increased (P<0.05),the double calving rate of the subcutaneous injection of melatonin group was significantly increased (P<0.05),the pregnancy rate and calving rate of the first mating were significantly increased (P<0.05),and the pregnancy rate and calving rate of the second mating were not significantly different (P>0.05).The results of serum hormone showed that compared with control group,the levels of melatonin,LH and E₂ in the serum of the subcutaneous injection melatonin group were significantly increased (P<0.05),the content of P₄ in the pregnant cows with subcutaneous injection melatonin was significantly increased after 35 d of pregnancy examination (P<0.05).The results showed that subcutaneous injection of melatonin could improve the pregnancy rate,calving rate and serum LH,E₂ and P₄ contents of Holstein dairy cows,indicating that subcutaneous injection of melatonin could promote oocyte maturation and ovulation,and improve the pregnancy rate of breeding.

Key words: melatonin; Holstein dairy cow; synchronous-ovulation and timed artificial insemination; gonadotropin-releasing hormone; pregnancy rate

CLC Number:

S823

WU Hao, YAO Songyang, SONG Yukun, MA Hui, GUO Gang, WANG Tiankun, LI Xiubo, LU Yongqiang, ZHANG Lu, LIU Guoshi. Effects of Melatonin on Pregnancy Rate and Reproductive Hormone in Holstein Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2523-2529.

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Effects of Melatonin on Pregnancy Rate and Reproductive Hormone in Holstein Dairy Cows

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