China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (1): 125-133.doi: 10.16431/j.cnki.1671-7236.2023.01.013

• Nutrition and Feed • Previous Articles     Next Articles

Effect of Supplementation with L-arginine on Pregnancy Rate and Plasma Parameters of Re-service Thoroughbred Horses

ZANG Changjiang1, LIU Baoxuan1, YUAN Dong2, LI Xiaobin1, LI Fengming1, CHEN Kaixu1, MIREGULI·Yimamu1, YANG Kailun1   

  1. 1. Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Changji Jiyuan Animal Husbandry Co., Ltd., Changji 831100, China
  • Received:2022-06-16 Online:2023-01-05 Published:2023-01-04

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Abstract: 【Objective】 The purpose of the experiment was to study the effect of supplementation with L-arginine on pregnancy rate and plasma parameters of re-service thoroughbred horses.【Method】 14 re-service thoroughbred horses were randomly divided into 2 groups, 7 in each group(n=7), as the control group and L-arginine group.The control group was fed with basic diet, and the L-arginine group was added with 50 g/d L-arginine on the basis of basic diet.The pre feeding period was 7 d, and the trial period was 30 d.When the returning mare was in estrus, L-arginine was supplemented on the day after the first artificial insemination.At the end of mating, the pregnancy was examined by rectum B-ultrasound, and the pregnancy rate was calculated.Blood was collected from the jugular vein of mares at 0, 15 and 30 d respectively.The activities of total nitric oxide synthase (TNOS), endothelial nitric oxide synthase (eNOS) and the contonts of nitric oxide (NO), vascular endothelial growth factor (VEGF), growth hormone (GH), insulin-like growth factor(IGF) were measured.【Result】 Compared with the control group, the pregnancy rate of the L-arginine group was significantly increased (P<0.05).There was no significant difference in plasma TNOS, eNOS, NO, ODC, IGF-Ⅰ, IGF-Ⅱ and VEGF levels/activities between the two groups at 0, 15 and 30 d (P>0.05).On the 30th day of the test, the plasma ammonia (NH3) and urea nitrogen (UN) contents in the L-arginine group decreased by 13.49% and 26.91% respectively compared with the control group (P<0.05).Compared with the control group, the plasma GH content on the 30th day of the test was significantly increased by 50.26% in L-arginine group (P<0.01).And there was no significant difference in the contents of IgA, IgM and IgG of mares on the 0th, 15th, and 30th days of the test between two groups (P>0.05).【Conclusion】 Feeding L-arginine to the re-service thoroughbred horses could significantly increase the pregnancy rate and change the contents of UN, NH3 and GH in the circulating plasma of the mares.L-arginine could be used as a nutritional regulator to improve the pregnancy rate of thoroughbred mares.

Key words: L-arginine; mare; pregnancy rate; plasma parameters

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