延边黄牛MYH3和MYH8基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.06.025

Abstract

Abstract: The purpose of this study was to investigate the association of the polymorphisms of myosin heavy chain 3 (MYH3) and myosin heavy chain 8 (MYH8) gene with growth traits in Yanbian Yellow cattle.In this study,the genetic variation of MYH3 and MYH8 genes were analyzed by DNA direct sequencing method and high resolution melting curve in 120 heads of Yanbian Yellow cattle,and the correlation analysis was conducted based on the growth character data of Yanbian Yellow cattle.The results showed that a T>G mutation at locus 29602748 of the MYH3 gene was detected,containing two alleles:T and G,and three genotypes:TG,TT and GG.One C>T mutation at locus 29406042 of MYH8 gene was detected,in which there were two alleles:C and T,and three genotypes:CT,CC and TT.Correlation analysis showed that the T>G mutation at locus 29602748 of MYH3 gene significantly affected the height at hip cross and hip width of Yanbian Yellow cattle at 24 months age,that the height at hip cross of GG genotype was significantly higher than TT genotype (P<0.05),and the hip width was significantly higher than TT and TG genotypes (P<0.05).The C>T mutation at locus 29406042 of MYH8 gene significantly affected the chest circumference and body weight of Yanbian Yellow cattle at 24 months age,indicating that the body weight of CT and TT genotypes were significantly higher than CC genotype (P<0.05).Moreover,The chest circumference of TT genotype was significantly higher than CC genotype (P<0.05).In summary,the results of this study indicated that the polymorphisms of MYH3 and MYH8 genes were related to some growth traits of Yanbian Yellow cattle and could be used as candidate genes for marker-assisted selection in modern beef cattle breeding.

Key words: Yanbian Yellow cattle; MYH3; MYH8; SNP; growth traits

CLC Number:

S813.3

TIAN Quan, WANG Sihan, LIU Lei, ZHAO Yi, LIU Haixing, JIN Huazi, LI Zhongshu, ZHANG Junzheng, JI Shuang. Analysis of MYH3 and MYH8 Genes Polymorphisms and Their Correlation with Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2122-2128.

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Analysis of MYH3 and MYH8 Genes Polymorphisms and Their Correlation with Growth Traits in Yanbian Yellow Cattle

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